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Insight into the evolution of antibiotic resistance genes and microbial community during spiramycin fermentation residue composting process after thermally activated peroxydisulfate pretreatment
Journal of Hazardous Materials ( IF 13.6 ) Pub Date : 2021-09-21 , DOI: 10.1016/j.jhazmat.2021.127287
Gang Wang 1 , Huiling Liu 2 , Picheng Gong 1 , Jing Wang 1 , Xiaohu Dai 2 , Peng Wang 1
Affiliation  

Previous research has been demonstrated that the residual unextracted antibiotics in spiramycin fermentation residue (SFR) could be efficiently removed by thermally activated peroxodisulfate (TAP) pretreatment, indicating the improvement of biodegradability. This study aimed to investigate the effect of TAP pretreatment on the succession of bacterial community and fate of antibiotic resistance genes (ARGs) during SFR composting. Results indicated that TAP pretreatment increased the composting temperature and promoted the decomposition of organic matters. Furthermore, TAP pretreatment could increase bacterial alpha diversity and significantly reduce the relative abundance of ARGs (1.13–1.75 times) and mobile genetic elements (MGEs) (1.13–1.32 times) after composting. The compost of pretreated SFR by TAP could reduce the enrichment of ARGs and MGEs in the bacterial community, especially the rRNA methylase genes of ermB (4–142-folds). Redundancy analysis showed that Actinobacteria, Bacteroidetes, Proteobacteria and horizontal gene transfer mediated by MGEs (intI1) was positively related to the changes in ARGs (accounted for 97.4%). Network analysis showed that Firmicutes was the main bacterial hosts of ARGs and MGEs. These findings demonstrated that TAP pretreatment combined composting was a promising strategy for SFR safe treatment and disposal that could reduce the proliferation and transfer of ARGs.



中文翻译:

热活化过硫酸盐预处理后螺旋霉素发酵渣堆肥过程中抗生素抗性基因和微生物群落的演变

先前的研究表明,螺旋霉素发酵残渣(SFR)中残留的未提取抗生素可以通过热活化过二硫酸盐(TAP)预处理有效去除,表明生物降解性的提高。本研究旨在探讨 TAP 预处理对 SFR 堆肥过程中细菌群落演替和抗生素抗性基因 (ARGs) 命运的影响。结果表明,TAP预处理提高了堆肥温度,促进了有机物的分解。此外,TAP预处理可以增加细菌的α多样性,并显着降低堆肥后ARGs(1.13-1.75倍)和移动遗传元件(MGEs)(1.13-1.32倍)的相对丰度。ermB(4-142 倍)。冗余分析表明,MGEs(intI1 )介导的放线菌拟杆菌变形菌和水平基因转移与ARGs的变化呈正相关(占97.4%)。网络分析表明,厚壁菌门是 ARGs 和 MGEs 的主要细菌宿主。这些发现表明,TAP 预处理联合堆肥是一种有前途的 SFR 安全处理和处置策略,可以减少 ARGs 的扩散和转移。

更新日期:2021-09-29
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