Globally, Sweet potato feathery mottle virus (SPFMV) and Sweet potato chlorotic stunt virus (SPCSV) occur frequently and in combination cause sweetpotato virus disease (SPVD). Many viral diseases are economically important and negatively impact the production and movement of germplasm across regions. Rapid detection of viruses is critical for effective control. Detection and quantification of viruses directly from sweetpotato remains a challenge. Current diagnostic tests are not sensitive enough to reliably detect viruses directly from the plant or require expensive laboratory equipment and expertise to perform. We developed a simple and rapid loop‐mediated isothermal amplification (LAMP) assay for the detection of SPFMV, SPCSV and begomoviruses related to sweet potato leaf curl virus (SPLCV). Laboratory validation recorded 100 % diagnostic sensitivity for all the three viruses. The LAMP assays were customized for field testing using a lyophilized thermostable isothermal master mix in a ready-to-use form that required no cold chain. The average time to positivity (TTP) was: SPFMV 5−30 min, SPCSV 15–43 min s and begomoviruses 28−45 mins. LAMP on-site testing results were comparable to PCR and RT-PCR confirmatory laboratory tests. The LAMP assay is a powerful tool for rapid sweetpotato virus detection at a reasonable cost and thus could serve as quality control systems for planting materials.

在全球范围内，甘薯羽状斑驳病毒（SPFMV）和甘薯褪绿矮化病毒（SPCSV）频繁发生，并共同导致甘薯病毒病（SPVD）。许多病毒性疾病具有重要的经济意义，并对种质的跨地区生产和流动产生负面影响。快速检测病毒对于有效控制至关重要。直接从甘薯中检测和量化病毒仍然是一个挑战。当前的诊断测试不够灵敏，无法可靠地直接从工厂检测病毒，或者需要昂贵的实验室设备和专业知识才能执行。我们开发了一种简单快速的环介导等温扩增 (LAMP) 检测方法，用于检测与甘薯卷叶病毒 (SPLCV) 相关的 SPFMV、SPCSV 和贝戈莫病毒。实验室验证记录了所有三种病毒的 100% 诊断敏感性。LAMP 检测是为现场测试定制的，使用冻干的热稳定等温预混液，即用型，无需冷链。平均阳性时间 (TTP) 为：SPFMV 5-30 分钟，SPCSV 15-43 分钟和贝戈莫病毒 28-45 分钟。LAMP 现场测试结果与 PCR 和 RT-PCR 确认性实验室测试相当。LAMP 检测是以合理的成本快速检测甘薯病毒的有力工具，因此可以作为种植材料的质量控制系统。SPCSV 15-43 分钟和贝戈莫病毒 28-45 分钟。LAMP 现场测试结果与 PCR 和 RT-PCR 确认性实验室测试相当。LAMP 检测是以合理的成本快速检测甘薯病毒的有力工具，因此可以作为种植材料的质量控制系统。SPCSV 15-43 分钟和贝戈莫病毒 28-45 分钟。LAMP 现场测试结果与 PCR 和 RT-PCR 确认性实验室测试相当。LAMP 检测是以合理的成本快速检测甘薯病毒的有力工具，因此可以作为种植材料的质量控制系统。