Superovulation and embryo transfer techniques are important methods in cattle breeding. Combined with traditional superovulation protocols, immunization against inhibin can further improve follicular development and embryo yield. The aim of this study is to determine the efficacy of immunization against inhibin in improving the fertility of cattle through meta-analysis and to provide better clinical veterinary practice guidance. Three English databases (PubMed, EMBASE, Web of Science) were searched for research articles of immunizations against inhibin influence on cattle fertility. Literature screening, data extraction, and meta-analysis were conducted in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. In addition, the Systematic Review Center for Laboratory animal Experimentation (SYRCLE) risk-of-bias (RoB) tool was used to assess the risk of bias of the included animal studies. Potentially relevant studies (317) were identified, and finally 14 eligible studies (all in English) were included. The results of meta-analysis revealed that immunization against inhibin has significant effects on improving the number of ovulations [mean difference (MD) = 0.44, 95% confidence interval (CI) = (0.31, 0.56)], embryos and unfertilized ova [MD = 4.51, 95% CI = (2.28, 6.74)], follicles of the three size categories, the incidence of multiple ovulations [OR = 22.50, 95% CI = (8.13, 62.27)], and the conception rate [OR = 2.36, 95% CI = (1.26, 4.40)]. Moreover, it improved the production of embryos [grades 1 embryos: MD = 3.84, (3.54, 4.15); grade 2 embryos: MD = −0.73, (−0.89, −0.57); grade 3 embryos: MD = −0.50, (−0.75, −0.25); degenerated embryos: MD = 1.16, (−0.51, 2.82); transferable embryos: MD = 2.67, (2.03, 3.31)] and the number of corpora lutea [MD = 1.25, 95% CI = (0.79, 1.71)]. In the above indicators, the differences between the two groups were statistically significant (all p < 0.0001). Additionally, according to the quality evaluation results, the risk of bias in the included studies is relatively high. The quality evaluation of the results of the included studies showed that the risk of bias mainly concentrated in the selective, performance, detection, and reporting of bias aspects.

超数排卵和胚胎移植技术是牛育种的重要方法。结合传统的超排卵方案，针对抑制素的免疫接种可以进一步提高卵泡发育和胚胎产量。本研究的目的是通过荟萃分析确定针对抑制素的免疫接种在提高牛的生育能力方面的功效，并提供更好的临床兽医实践指导。搜索了三个英文数据库（PubMed、EMBASE、Web of Science）中关于免疫抑制素对牛生育能力影响的研究文章。文献筛选、数据提取和荟萃分析是根据系统评价和荟萃分析的首选报告项目 (PRISMA) 指南进行的。此外，实验动物实验系统审查中心 (SYRCLE) 的偏倚风险 (RoB) 工具用于评估纳入的动物研究的偏倚风险。确定了潜在的相关研究 (317)，最终纳入了 14 项符合条件的研究（全部为英文）。荟萃分析结果显示，免疫抑制素对提高排卵数[平均差（MD）=0.44，95%置信区间（CI）=（0.31，0.56）]、胚胎和未受精卵[MD = 4.51, 95% CI = (2.28, 6.74)]，三种大小的卵泡，多次排卵的发生率 [OR = 22.50, 95% CI = (8.13, 62.27)]，受孕率 [OR = 2.36 , 95% CI = (1.26, 4.40)]。此外，它提高了胚胎的产量[1 级胚胎：MD = 3.84, (3.54, 4.15); 2 级胚胎：MD = -0.73, (-0. 89, -0.57); 3 级胚胎：MD = -0.50, (-0.75, -0.25); 退化胚胎：MD = 1.16, (-0.51, 2.82); 可移植胚胎：MD = 2.67, (2.03, 3.31)] 和黄体数量 [MD = 1.25, 95% CI = (0.79, 1.71)]。上述指标中，两组差异有统计学意义（均磷< 0.0001)。此外，从质量评价结果来看，纳入研究的偏倚风险较高。对纳入研究结果的质量评价表明，偏倚风险主要集中在偏倚的选择、性能、检测和报告等方面。