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Chromosomal Differentiation of Deschampsia (Poaceae) Based on Four Satellite DNA Families
Frontiers in Genetics ( IF 3.7 ) Pub Date : 2021-09-21 , DOI: 10.3389/fgene.2021.728664
María Laura González 1 , Jorge Oscar Chiapella 2 , Juan Domingo Urdampilleta 1
Affiliation  

Diverse families of satellite DNA (satDNA) were detected in heterochromatin regions of Deschampsia. This kind of repetitive DNA consists of tandem repeat sequences forming big arrays in genomes, and can contribute to lineages differentiation. The differentiation between types of satDNA is related to their sequence identity, the size and number of monomers forming the array, and their chromosomal location. In this work, four families of satDNA (D2, D3, D12, D13), previously isolated by genomic analysis, were studied on chromosomal preparations of 12 species of Deschampsia (D. airiformis, D. antarctica, D. cespitosa, D. cordillerarum, D. elongata, D. kingii, D. laxa, D. mendocina, D. parvula, D. patula, D. venustula, and Deschampsia sp) and one of Deyeuxia (D. eminens). Despite the number of satDNA loci showing interspecific variation, the general distribution pattern of each satDNA family is maintained. The four satDNA families are AT-rich and associated with DAPI + heterochromatin regions. D2, D3, and D12 have mainly subterminal distribution, while D13 is distributed in intercalary regions. Such conservation of satDNA patterns suggests a not random distribution in genomes, where the variation between species is mainly associated with the array size and the loci number. The presence of satDNA in all species studied suggests a low genetic differentiation of sequences. On the other hand, the variation of the distribution pattern of satDNA has no clear association with phylogeny. This may be related to high differential amplification and contraction of sequences between lineages, as explained by the library model.



中文翻译:

基于四个卫星DNA家族的禾本科(禾本科)染色体分化

在异染色质区域检测到不同的卫星 DNA (satDNA) 家族 德尚. 这种重复 DNA 由串联重复序列组成,在基因组中形成大阵列,有助于谱系分化。satDNA 类型之间的区别与其序列同一性、形成阵列的单体的大小和数量以及它们的染色体位置有关。在这项工作中,之前通过基因组分析分离的四个卫星 DNA 家族(D2、D3、D12、D13)对 12 种植物的染色体制备物进行了研究。德尚 (D.airiformis, D.南极洲, D. cespitosa, D.cordillerarum, D. elongata, 金龟子, D. 松弛, D. 门多西纳, D. parvula, D. patula, D.小静脉,地香属) 和其中之一 德约夏 (D. eminens)。尽管 satDNA 基因座的数量显示出种间变异,但每个 satDNA 家族的一般分布模式仍保持不变。四个 satDNA 家族富含 AT,并与 DAPI + 异染色质区域相关。D2、D3、D12主要为亚末端分布,D13分布于居间区。satDNA 模式的这种保守性表明基因组中的分布不是随机的,其中物种之间的差异主要与阵列大小和基因座数有关。所研究的所有物种中都存在卫星 DNA 表明序列的遗传差异较低。另一方面,satDNA 分布模式的变化与系统发育没有明确的关联。这可能与谱系之间序列的高度差异扩增和收缩有关,

更新日期:2021-09-21
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