A core-shell AuNRs@Ag-enhanced and magnetic separation-assisted surface-enhanced Raman spectroscopy (SERS) immunosensing platform was developed for rapid, accurate and sensitive detection of amantadine (AMD) contaminant in animal-derived foods. This SERS immunosensing platform works in a competitive strategy, which used the 4-mercaptobenzoic acid (4-MBA) labeled AuNRs@Ag with AMD-BSA immobilized as a signal probe and magnetic beads (MBs) with mAbs covalently attached to the surface as a capture probe. It was found that the synthesized core-shell AuNRs@Ag could significantly enhance the SERS signal of the reporter 4-MBA (EF: 2.91 × 10⁷), and the coating of Ag shell effectively avoided the shedding of 4-MBA, thereby improving the sensitivity and stability of SERS analysis. The use of MBs not only overcomes the shortcoming of mAbs inactivation, but also simplifies the detection procedure, improving the detection efficiency. This SERS immunosensing platform was demonstrated to have a linear response in a wide AMD concentration range of 0.01–50 μg/L with high sensitivity (limit of detection (LOD): 0.0038 μg/L) and specificity. For actual animal-derived foods (chicken, milk, egg), acceptable recoveries of AMD (82.0–106.0%) at three spiked levels (0.5, 5, 50 μg/kg) were obtained with relative standard deviation (RSD, n = 5) ranging from 4.7% to 9.6%. The whole detection procedure can be completed within 30 min. These results demonstrated that the proposed SERS immunosensing platform was reliable and sensitive in the quantitative analysis of AMD and could be applied as an effective tool for rapid screening for AMD-contaminated foods.

开发了核壳 AuNRs@Ag 增强和磁分离辅助的表面增强拉曼光谱 (SERS) 免疫传感平台，用于快速、准确和灵敏地检测动物源性食品中的金刚烷胺 (AMD) 污染物。该 SERS 免疫传感平台采用竞争策略，使用 4-巯基苯甲酸 (4-MBA) 标记的 AuNRs@Ag，固定有 AMD-BSA 作为信号探针，并使用与表面共价连接的 mAb 作为磁珠 (MB)捕获探针。发现合成的核壳 AuNRs@Ag 可以显着增强报告基因 4-MBA 的 SERS 信号 ( EF : 2.91 × 10 ⁷)，并且Ag壳的涂层有效地避免了4-MBA的脱落，从而提高了SERS分析的灵敏度和稳定性。MBs的使用不仅克服了mAbs失活的缺点，而且简化了检测程序，提高了检测效率。该 SERS 免疫传感平台被证明在 0.01-50 μg/L 的宽 AMD 浓度范围内具有线性响应，具有高灵敏度（检测限 (LOD)：0.0038 μg/L）和特异性。对于实际的动物源性食品（鸡肉、牛奶、鸡蛋），在三个加标水平（0.5、5、50 μg/kg）下获得了可接受的 AMD 回收率（82.0-106.0%），并具有相对标准偏差（RSD，n = 5） ) 范围从 4.7% 到 9.6%。整个检测过程可在30分钟内完成。