A portable imprinted electrochemical sensor with a self-driven polyethylene terephthalate (PET) chip was developed for the fast detection of Salmonella. A chip with a siphon inlet connecting the reaction chamber and a capillary connecting the reaction chamber to the press chamber was innovatively designed to achieve self-drive. Based on siphonage and hydrophilicity, the liquid entered the reaction chamber automatically, and quantitative sampling was completed when the liquid reached the capillary. Waste liquid was discharged from the reaction chamber by pressing the press chamber. The self-driven PET electrode chip was prepared by screen-printing and laser-cutting, and Salmonella-imprinted film was then prepared on the chip working electrode via electropolymerization. Characterization studies of the modification of the working electrode were then carried out via SEM, AFM, water contact angle and electrochemistry. The detection of Salmonella was performed by differential pulse voltammetry within the range of 3.0 × 10² to 3.0 × 10⁷ CFU mL⁻¹, and the limit of detection was 100 CFU mL⁻¹ (S/N = 3). The selectivity of the proposed sensor was then examined via the use of interfering bacteria, including E. coli, L. monocytogenes, and P. aeruginosa. The practical application value of the sensor was demonstrated by a recovery rate of 92–109% and a relative standard deviation of less than 2.96% in the detection of Salmonella in milk and orange juice samples. In summary, a self-driven PET chip-based imprinted electrochemical sensor for the detection of Salmonella within 20 min was developed, and has the potential to monitor and quickly control Salmonella contamination in food processing and food circulation.

开发了一种带有自驱动聚对苯二甲酸乙二醇酯 (PET) 芯片的便携式印迹电化学传感器，用于快速检测沙门氏菌。创新设计了带有连接反应室的虹吸管入口和连接反应室和压室的毛细管的芯片，以实现自驱动。基于虹吸和亲水性，液体自动进入反应室，当液体到达毛细管时完成定量取样。通过挤压加压室，废液从反应室排出。通过丝网印刷和激光切割制备自驱动PET电极芯片，沙门氏菌然后通过电聚合在芯片工作电极上制备-压印膜。然后通过SEM、AFM、水接触角和电化学对工作电极的改性进行表征研究。沙门氏菌检测采用差分脉冲伏安法，检测范围为3.0×10 ²～3.0×10 ⁷ CFU mL ^-1，检测限为100 CFU mL ^-1 (S/N=3)。然后通过使用干扰细菌（包括大肠杆菌、单核细胞增生李斯特菌和铜绿假单胞菌）来检查所提出的传感器的选择性. 该传感器在牛奶和橙汁样品中沙门氏菌检测中的回收率为92-109%，相对标准偏差小于2.96%，证明了该传感器的实际应用价值。总之，开发了一种基于自驱动 PET 芯片的印迹电化学传感器，可在 20 分钟内检测沙门氏菌，具有监测和快速控制食品加工和食品流通中沙门氏菌污染的潜力。