The current works report the bio-efficacy of Pimenta dioica leaf derived silver nanoparticles (Pd@AgNPs) and leaf extract obtained trough different solvents against the larvae of malaria, filarial and dengue vectors. Synthesis of silver nanoparticles (AgNPs) was done by adding 10 ml of P. dioica leaf extract into 90 ml of 1 mM silver nitrate solution, a slow colour change was observed depicting the formation of AgNPs. Further, Pd@AgNPs was confirmed through Ultraviolet–visible spectroscopy which exhibited characteristic absorption peak at 422 nm wavelength. X-ray diffraction and selected area electron diffraction analysis confirmed monodispersed and crystalline nature of Pd@AgNPs with 32 nm an average size. Scanning electron microscopy and transmission electron microscopy showed the most of Pd@AgNPs were spherical and triangular in shape and energy-dispersive X-ray spectroscopy revealed silver elemental nature of nanoparticles. Zeta potential of Pd@AgNPs is highly negative which confirmed its stable nature. Pd@AgNPs showed prominent absorption peaks at 1015, 1047, 1243, 1634, 2347, 2373, 2697 and 3840 cm⁻¹ which are corresponding to following compounds polysaccharides, carboxylic acids, water, alcohols, esters, ethers, amines, amides and phenol, respectively as reported by Fourier-transform infrared spectroscopy analysis. Gas chromatography–mass spectrometry and Liquid chromatography–mass spectrometry analysis revealed 39 and 70 compounds, respectively, which might be contributed for bio-reduction, capping, stabilization and larvicidal behavior of AgNPs. A comparable lethality (LC₅₀ and LC₉₀) was observed in case of Pd@AgNPs over leaf extract alone. The potential larvicidal activity of Pd@AgNPs was observed against the larvae of Aedes aegypti,(LC_50, 2.605; LC_90, 5.084 ppm) Anopheles stephensi (LC_50, 3.269; LC_90, 7.790 ppm) and Culex quinquefasciatus (LC_50, 5.373; LC_90, 14.738 ppm without affecting non-targeted organism, Mesocyclops thermocyclopoides after 72 hr of exposure. This study entails green chemistry behind synthesis of AgNPs which offers effective technique for mosquito control and other therapeutic applications.

目前的工作报告了二甘香叶衍生的银纳米颗粒 (Pd@AgNPs) 和通过不同溶剂获得的叶提取物对疟疾、丝虫和登革热病媒幼虫的生物功效。银纳米粒子 (AgNPs) 的合成是通过添加 10 ml 的P. dioica完成的叶提取物放入 90 ml 1 mM 硝酸银溶液中，观察到缓慢的颜色变化，描绘了 AgNPs 的形成。此外，通过紫外-可见光谱证实了 Pd@AgNPs，其在 422 nm 波长处表现出特征吸收峰。X 射线衍射和选区电子衍射分析证实了 Pd@AgNPs 的单分散和结晶性质，平均尺寸为 32 nm。扫描电子显微镜和透射电子显微镜显示大多数 Pd@AgNPs 呈球形和三角形，能量色散 X 射线光谱显示纳米粒子的银元素性质。Pd@AgNPs 的 Zeta 电位是高度负的，这证实了它的稳定性。Pd@AgNPs 在 1015、1047、1243、1634、2347、2373、2697 和 3840 cm ^{-1处显示出显着的吸收峰}如傅里叶变换红外光谱分析所报道的，它们分别对应于以下化合物多糖、羧酸、水、醇、酯、醚、胺、酰胺和苯酚。气相色谱-质谱和液相色谱-质谱分析分别揭示了 39 和 70 种化合物，它们可能有助于 AgNPs 的生物还原、封端、稳定和杀幼虫行为。Pd@AgNPs 在单独的叶子提取物上观察到相当的致死率（LC ₅₀和 LC _{90 ）。}观察到 Pd@AgNPs 对埃及伊蚊幼虫的潜在杀幼虫活性（LC _50, 2.605; LC _90, 5.084 ppm）斯蒂芬按蚊(LC _50, 3.269; LC _90, 7.790 ppm) 和Culex quinquefasciatus (LC _50, 5.373; LC _90, 14.738 ppm，暴露 72 小时后不影响非目标生物，Mesocyclops thermocyclopoides 。这项研究需要在 AgNPs 合成背后的绿色化学它为蚊子控制和其他治疗应用提供了有效的技术。