The Kingdom of Saudi Arabia thrives with great plant diversity, including rare plants of the family Asphodelaceae that have multiple benefits and are still being studied. Aloe shadensis is one of these plants that must be preserved and documented in its natural environment. The most appropriate molecular approach currently approved for documentation is the sequencing of some genomic markers. The current study is the first to use genomic markers to record this rare plant. In this study, the plastid genes matK (Maturase K), rbcL (Ribulose-bisphosphate carboxylase/oxygenase large subunit), and the nuclear region ITS (Internal transcribed spacer) were used to reveal their efficiency in identifying the plant under study. This study is the first to deal with this plant and document it using these genetic markers. The study showed a promising result concerning identifying the sequence of the matK gene and ITS region, while the rbcL gene did not give a good indicator through the used primers. The obtained sequences of the matK gene and the ITS region were determined through two different sets of primers in each case then deposited in GenBank. The evolutionary relatedness of Aloe shadensis was established with the different species of Aloe. The study showed that the closest species is Aloe vera with a similarity of more than 99 %. The study concludes with the possibility of using these genes to correctly identify, distinguish and document the species of Aloe shadensis.

沙特阿拉伯王国以丰富的植物多样性蓬勃发展，包括具有多种益处且仍在研究中的天竺葵科稀有植物。芦荟是必须在自然环境中保存和记录的植物之一。目前批准用于记录的最合适的分子方法是一些基因组标记的测序。目前的研究是第一次使用基因组标记来记录这种稀有植物。在本研究中，质体基因matK (Maturase K)、rbcL（核酮糖二磷酸羧化酶/加氧酶大亚基）和核区 ITS（内部转录间隔区）用于揭示它们在鉴定所研究植物中的效率。这项研究是第一个处理这种植物并使用这些遗传标记对其进行记录的研究。该研究显示了关于鉴定matK基因和 ITS 区域的序列的有希望的结果，而rbcL基因通过使用的引物没有给出很好的指示。获得的matK基因和ITS区域的序列在每种情况下通过两组不同的引物确定，然后保存在GenBank中。芦荟的进化亲缘关系是建立在不同种类的芦荟上的. 研究表明，最接近的物种是芦荟，相似度超过 99%。该研究以使用这些基因正确识别、区分和记录芦荟物种的可能性而结束。