Abstract

In this study, ethylenediaminetetraacetic acid (EDTA) disodium was first chosen as catalyst to convert psoralenoside (PO) to psoralen (PSO) for increasing the extraction yield of PSO. An efficient continuous system for synchronous transformation and extraction of PSO from fig leaves applying microwave-assisted EDTA disodium (MAE-EDTA) was developed. The optimal MAE-EDTA condition was obtained: EDTA disodium concentration of 0.07 mol·L⁻¹, ethanol volume fraction of 56%, extraction time of 16 min, and extraction temperature of 70 °C by single factor experiments and response surface method (RSM). Under the optimal condition, the yield of PSO reached 27.24 mg·g⁻¹. Compared with microwave-assisted ethanol extraction (MAE) and reflux extraction (RE), the yield of PSO by MAE-EDTA is 2.03-fold higher than RE and 1.70-fold higher than MAE. Therefore, MAE-EDTA is an efficient method for extracting PSO from fig leaves, and it might provide references for the extraction of PSO from other medicinal plants.

摘要

在这项研究中，首先选择乙二胺四乙酸 (EDTA) 二钠作为催化剂将补骨脂苷 (PO) 转化为补骨脂素 (PSO)，以提高 PSO 的提取率。应用微波辅助 EDTA 二钠 (MAE-EDTA) 开发了一种从无花果叶中同步转化和提取 PSO 的高效连续系统。通过单因素实验和响应面法（RSM ）得到最佳MAE-EDTA条件：EDTA二钠浓度0.07 mol·L ⁻¹，乙醇体积分数56%，提取时间16 min，提取温度70 ℃ ). 在最佳条件下，PSO的产率达到27.24 mg·g ⁻¹. 与微波辅助乙醇提取 (MAE) 和回流提取 (RE) 相比，MAE-EDTA 的 PSO 产量比 RE 高 2.03 倍，比 MAE 高 1.70 倍。因此，MAE-EDTA是一种从无花果叶中提取PSO的有效方法，可为其他药用植物中PSO的提取提供参考。