Efficient design for bone tissue engineering requires an understanding of the appropriate selection of biomimetic natural or synthetic materials and scalable fabrication technologies. In this research, poly (3-hydroxybutyrate) (PHB) and starch (5-15 wt%) as biological macromolecules were used to fabricate novel biomimetic scaffolds by electrospinning method. SEM results of electrospun scaffolds revealed bead-free nanofibers and three-dimensional homogenous structures with highly interconnected pores. Results of FTIR and Raman demonstrated that there were hydrogen bonds between the two polymers. The tensile strength of scaffolds was significantly improved by adding starch up to 10 wt%, from 3.05 to 15.54 MPa. In vitro degradation and hydrophilicity of the scaffolds were improved with the presence of starch. The viability and proliferation of MG-63 cells and alkaline phosphatase (ALP) activity were remarkably increased in the PHB-starch scaffolds compared to the PHB and control samples. The mineralization and calcium deposition of MG-63 cells were confirmed by alizarin red staining. It is concluded that PHB/starch electrospun scaffold could be a good candidate for bone tissue engineering applications.

Efficient design for bone tissue engineering requires an understanding of the appropriate selection of biomimetic natural or synthetic materials and scalable fabrication technologies. 在这项研究中，聚（3-羟基丁酸酯）（PHB）和淀粉（5-15 wt%）作为生物大分子被用来通过静电纺丝方法制造新型仿生支架。电纺支架的 SEM 结果显示无珠纳米纤维和具有高度互连孔的三维均匀结构。FTIR 和拉曼的结果表明两种聚合物之间存在氢键。通过添加高达 10 wt% 的淀粉，支架的拉伸强度显着提高，从 3.05 到 15.54 MPa。体外淀粉的存在改善了支架的降解和亲水性。与 PHB 和对照样品相比，PHB-淀粉支架中 MG-63 细胞的活力和增殖以及碱性磷酸酶 (ALP) 活性显着增加。通过茜素红染色证实MG-63细胞的矿化和钙沉积。得出的结论是，PHB/淀粉电纺支架可能是骨组织工程应用的良好候选者。