Xylooligosaccharides (XOs) are xylose oligomers that stimulate the growth of probiotic microorganisms and the production of substances with antimicrobial activity, such as bacteriocins. XOs are of great interest to the food and pharmaceutical industries and represent a promising product for the valorization of sugarcane bagasse in a biorefinery context. Here, the whole XOs production chain from sugarcane bagasse was studied, starting with biomass pretreatment, enzymatic hydrolysis of the hemicellulose fraction, purification and evaluation of nutritional properties of the final product. Hydrothermal and organosolv pretreatments stood out among four pretreatment alternatives, solubilizing hemicellulose mainly in the form of oligomers. Hydrothermal conditions for the highest XOs yield were determined (185 °C, 10 min, 1:10 solid:liquid ratio). Enzymatic conversion to XOs using an endoxylanase reached app. 90 %, for both hydrothermal and organosolv liquors. Prebiotic and antimicrobial properties of the XOs obtained after hydrothermal and organosolv pretreatments were evaluated. Both XOs presented probiotic activity, but substantially superior results were achieved for the hydrothermal pretreatment. XOs liquors were further purified using adsorption on activated charcoal and the purified XOs liquor significantly increased the cell concentration of probiotic lactic bacteria (L. acidophilus, L. reuteri and L. rhamnosus). The presence of bacteriocins with antimicrobial activity against Bacillus subtilis was also detected in culture plates (40 AU/mL). Although still requiring further optimization of purification steps, the results provide important information for developing sugarcane biorefineries producing XOs.

低聚木糖 (XO) 是木糖低聚物，可刺激益生菌微生物的生长和具有抗菌活性的物质（如细菌素）的产生。XO 对食品和制药行业非常感兴趣，并且代表了一种在生物精炼环境中使甘蔗渣增值的有前途的产品。在这里，研究了来自甘蔗渣的整个 XOs 生产链，从生物质预处理、半纤维素部分的酶水解、纯化和最终产品的营养特性评估开始。水热和有机溶剂预处理在四种预处理替代方案中脱颖而出，主要以低聚物的形式溶解半纤维素。确定了最高 XO 产率的水热条件（185 °C，10 分钟，1:10 固液比）。使用内切木聚糖酶将酶促转化为 XO。90 %，用于水热和有机溶剂溶液。评估了水热和有机溶剂预处理后获得的 XO 的益生元和抗菌特性。两种 XO 都具有益生菌活性，但水热预处理取得了显着优越的结果。XOs 溶液通过活性炭吸附进一步纯化，纯化后的 XOs 溶液显着增加了益生菌乳酸菌的细胞浓度。但水热预处理取得了显着优越的结果。XOs 溶液通过活性炭吸附进一步纯化，纯化后的 XOs 溶液显着增加了益生菌乳酸菌的细胞浓度。但水热预处理取得了显着优越的结果。XOs 溶液通过活性炭吸附进一步纯化，纯化后的 XOs 溶液显着增加了益生菌乳酸菌的细胞浓度。嗜酸乳杆菌、罗伊氏乳杆菌和鼠李糖乳杆菌）。在培养板 (40 AU/mL) 中也检测到对枯草芽孢杆菌具有抗菌活性的细菌素的存在。尽管仍需要进一步优化纯化步骤，但结果为开发生产 XO 的甘蔗生物精炼厂提供了重要信息。