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Broadband surface plasmon resonance sensor for fast spectroscopic photoacoustic microscopy
Photoacoustics ( IF 7.9 ) Pub Date : 2021-09-21 , DOI: 10.1016/j.pacs.2021.100305
Fan Yang 1 , Guangdi Guo 1 , Shanguang Zheng 1 , Hui Fang 1 , Changjun Min 1 , Wei Song 1 , Xiaocong Yuan 1
Affiliation  

High-speed optical-resolution photoacoustic microscopy (OR-PAM), integrating the merits of high spatial resolution and fast imaging acquisition, can observe dynamic processes of the optical absorption-based molecular specificities. However, it remains challenging for the evaluation to morphological and physiological parameters that are closely associated with photoacoustic spectrum due to the inadequate ultrasonic frequency response of the routinely-employed piezoelectric transducer. By utilizing the galvanometer for fast optical scanning and our previously-developed surface plasmon resonance sensor as an unfocused broadband ultrasonic detector, high-speed spectroscopic photoacoustic imaging was accessed in the OR-PAM system, achieving an acoustic bandwidth of ∼125 MHz and B-scan rate at ∼200 Hz over a scanning range of ∼0.5 mm. Our system demonstrated the dynamic imaging of the moving phantoms’ structures and the simultaneous characterization of their photoacoustic spectra over time. Further, fast volumetric imaging and spectroscopic analysis of microanatomic features of a zebrafish eye ex vivo was obtained label-freely.

中文翻译:

用于快速光谱光声显微镜的宽带表面等离子体共振传感器

高速光学分辨率光声显微镜(OR-PAM)结合了高空间分辨率和快速成像采集的优点,可以观察基于光学吸收的分子特异性的动态过程。然而,由于常规使用的压电换能器的超声频率响应不足,对与光声光谱密切相关的形态和生理参数的评估仍然具有挑战性。通过利用检流计进行快速光学扫描,并利用我们之前开发的表面等离子体共振传感器作为未聚焦宽带超声波探测器,在 OR-PAM 系统中实现了高速光谱光声成像,实现了~125 MHz 的声学带宽和 B-扫描速率约为 200 Hz,扫描范围约为 0.5 毫米。我们的系统展示了移动模型结构的动态成像以及随时间变化的光声光谱的同步表征。此外,无需标记即可获得斑马鱼眼睛离体显微解剖特征的快速体积成像和光谱分析。
更新日期:2021-09-21
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