Background

Ocular hypertension is a major risk factor for glaucoma, which is characterized by the presence of ganglion cells, ganglion disease, and an irreversible reduction in the number of ganglion cells and axons. Although the anti-inflammatory and detoxifying effects of the maple tree (Acer palmatum Thunb.) leave extract are well known, it is unclear whether the extract can protect the eyes from ocular hypertension.

Objective

This study was conducted to investigate whether the alcohol extract from A. palmatum Thunb. leaves (KIOM-2015E) had a protective effect on eyes in an animal model of steroid-induced ocular hypertension. We investigated whether KIOM-2015E had a therapeutic effect on ocular diseases induced by elevated intraocular pressure (IOP) by reducing IOP.

Results

Ocular hypertension was induced in 9-week-old female C57BL/6 mice weighing 23–27 g with 4 weeks of steroid injection. After 4 weeks, 1 or 2 mg/mL of KIOM-2015E was instilled in the mice three times a day for 5 weeks. IOP was measured weekly, and the eyes were lysed after drug administration to investigate the changes in the histological parameters and the levels of anti-apoptotic proteins. IOP was elevated, OPN1SW levels were decreased in the retinal tissue, and GFAP levels were increased in the mice with induced ocular hypertension. IOP was lowered in a concentration-dependent manner in the mice treated with KIOM-2015E. KIOM-2015E was confirmed to have an inhibitory effect on the histological changes on the retina, activity of glial and astrocytic cells, and cell death.

Conclusions

Treatment with KIOM-2015E improved steroid-induced ocular hypertension. KIOM-2015E prevented retinal degeneration caused by increased ocular pressure by regulating protein levels and histological parameter expression in retina.

中文翻译：

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Acer palmatum Thunb 的保护作用。叶提取物对类固醇诱导的高眼压小鼠

背景

高眼压是青光眼的主要危险因素，其特征是神经节细胞的存在、神经节疾病以及神经节细胞和轴突数量的不可逆减少。虽然槭树（Acer palmatum Thunb.）叶提取物的抗炎和解毒作用众所周知，但尚不清楚该提取物是否可以保护眼睛免受高眼压。

客观的

进行这项研究是为了调查A的醇提取物是否。 掌楸 叶 (KIOM-2015E) 在类固醇诱导的高眼压动物模型中对眼睛有保护作用。我们研究了 KIOM-2015E 是否通过降低 IOP 对由升高的眼内压 (IOP) 引起的眼部疾病具有治疗作用。

结果

在 9 周大的雌性 C57BL/6 小鼠中，重 23-27 g，注射了 4 周的类固醇，诱发了高眼压。4 周后，每天 3 次向小鼠滴注 1 或 2 mg/mL KIOM-2015E，持续 5 周。每周测量眼压，给药后裂解眼睛以研究组织学参数和抗凋亡蛋白水平的变化。眼压升高，视网膜组织中的 OPN1SW 水平降低，诱导高眼压小鼠的 GFAP 水平升高。在用 KIOM-2015E 治疗的小鼠中，IOP 以浓度依赖性方式降低。KIOM-2015E 被证实对视网膜的组织学变化、胶质细胞和星形细胞的活性以及细胞死亡具有抑制作用。

结论

KIOM-2015E 治疗改善了类固醇引起的高眼压症。KIOM-2015E 通过调节视网膜中的蛋白质水平和组织学参数表达来防止由眼压升高引起的视网膜变性。