当前位置: X-MOL 学术ACS Food Sci. Technol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Determination of Deoxynivalenol in Wheat, Barley, Corn Meal, and Wheat-Based Products by Simultaneous Multisample Fluorescence Polarization Immunoassay Using a Portable Analyzer
ACS Food Science & Technology Pub Date : 2021-09-20 , DOI: 10.1021/acsfoodscitech.1c00244
Ayano Nakamura 1 , Mitsutoshi Aoyagi 2 , Mao Fukuyama 3 , Masatoshi Maeki 4 , Akihiko Ishida 4 , Hirofumi Tani 4 , Koji Shigemura 5 , Akihide Hibara 3 , Manabu Tokeshi 4, 6, 7
Affiliation  

With globalization, importing and exporting of agricultural products and foods have expanded, and food safety has become an important issue worldwide. Mycotoxin contamination of agricultural products and foods (processed foods) can occur anywhere, and it is desirable to develop a technology that can detect mycotoxin levels easily and quickly. By using a portable analyzer that can measure multiple samples simultaneously by combining a microfluidic device and a fluorescence polarization measurement system (a portable FP analyzer), we have successfully measured deoxynivalenol (DON), which is a common mycotoxin, easily and quickly by fluorescence polarized immunoassay (FPIA). The recovery rates of DON spiked in wheat, barley, cornmeal, flour, bread, pasta, thick wheat noodles (udon), and thin wheat noodles (somen) were between 86.3 and 113.2%, and the coefficient of variation was less than 8.5%. These FPIA results were in good agreement with results obtained by the official government-recognized method of liquid chromatography–mass spectrometry for DON analysis. The FP portable analyzer has great potential as an analytical tool that contributes to food safety.

中文翻译:

使用便携式分析仪通过同时多样品荧光偏振免疫分析法测定小麦、大麦、玉米粉和小麦制品中的脱氧雪腐镰刀菌烯醇

随着全球化的发展,农产品和食品的进出口规模不断扩大,食品安全已成为世界范围内的重要问题。农产品和食品(加工食品)的霉菌毒素污染可以发生在任何地方,因此需要开发一种可以轻松快速地检测霉菌毒素水平的技术。通过结合微流控装置和荧光偏振测量系统(便携式FP分析仪),使用可同时测量多个样品的便携式分析仪,我们成功地通过荧光偏振测量轻松快速地测量了常见的霉菌毒素脱氧雪腐镰刀菌烯醇(DON)。免疫分析(FPIA)。小麦、大麦、玉米面、面粉、面包、意大利面、粗麦面(乌冬面)和细麦面(somen ) 介于 86.3% 和 113.2% 之间,变异系数小于 8.5%。这些 FPIA 结果与政府认可的官方认可的液相色谱-质谱法用于 DON 分析的结果非常一致。FP 便携式分析仪作为有助于食品安全的分析工具具有巨大的潜力。
更新日期:2021-10-15
down
wechat
bug