Molecular barcoding is used to tag nucleic acids with sequence-encoded identifiers to distinguish between different samples or individual molecules. I describe a one-pot procedure for efficiently tagging linear dsDNA with molecular barcodes. This approach has potential utility for high-throughput in vitro biochemical experiments that require uniform and quantitative dsDNA tagging.

中文翻译：

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使用脱氧尿苷切除的高效线性 dsDNA 标记**

分子条形码用于用序列编码的标识符标记核酸，以区分不同的样品或单个分子。我描述了一种用分子条形码有效标记线性 dsDNA 的单锅程序。这种方法对于需要统一和定量 dsDNA 标记的高通量体外生化实验具有潜在的实用性。