In order to investigate the dietary requirement of Trachinotus ovatus for vitamin E (VE) and its effects on tissue polyunsaturated fatty acids (PUFA) reservation, a 56-day feeding trial was performed with five diets (D1–D5) supplemented with different levels of VE at 34.56, 47.35, 61.16, 91.06 and 144.88 mg VE/kg diet. A total of 375 fish (initial weight 13.40 ± 0.08 g) were randomly distributed equally into 15 sea cages and each diet in triplicate cages. After 8 weeks of feeding, fish fed diet D3 exhibited significantly higher weight gain (WG), serum superoxide dismutase and glutathione peroxidase activities and also had higher docosahexaenoic acid (DHA) and n-3 PUFA contents in the raw muscle than those of fish fed diets D1 and D5 (p < .05). Moreover, n-6 PUFA and n-3 PUFA contents in cooked muscle of D3 and D4 groups were significantly higher than those of other groups (p < .05). Besides, the contents of hepatic arachidonic acids, eicosapentaenoic acid, DHA, n-6 PUFA and n-3 PUFA in fish of D3 and D4 groups were also significantly higher than those of D1 group (p < .05). Correspondingly, the transcript levels of genes related to PUFA β-oxidation (cpt1 and pparα) and synthesis (fas and elovl5) were lower in D3 and D4 groups. Based on regression analysis for WG, the optimum dietary VE level was 90.75 mg/kg, which was consistent with the above results that fish fed diets D3 and D4 displayed better growth performance, antioxidant capacity and muscle PUFA stability, where the last suggesting an effect of dietary VE against PUFA β-oxidation.

中文翻译：

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日粮维生素E对金鲳幼鱼生长性能、抗氧化能力和脂质代谢的影响

为了研究卵形鲫鱼对维生素 E (VE) 的日粮需求及其对组织多不饱和脂肪酸 (PUFA) 保留的影响，对添加不同水平维生素 E 的五种日粮 (D1-D5) 进行了为期 56 天的喂养试验。 VE 为 34.56、47.35、61.16、91.06 和 144.88 mg VE/kg 饮食。将总共​​ 375 条鱼（初始重量 13.40 ± 0.08 g）随机平均分配到 15 个海水网箱中，每种饲料都放在一式三份的网箱中。喂食 8 周后，与喂食的鱼相比，喂食 D3 的鱼的体重增加（WG）、血清超氧化物歧化酶和谷胱甘肽过氧化物酶活性显着提高，生肌肉中的二十二碳六烯酸（DHA）和 n-3 PUFA 含量也更高。饮食 D1 和 D5 ( p < .05)。此外，D3和D4组熟肌中n-6 PUFA和n-3 PUFA含量显着高于其他组（p  < .05）。此外，D3和D4组鱼肝花生四烯酸、二十碳五烯酸、DHA、n-6 PUFA和n-3 PUFA的含量也显着高于D1组（p  < .05）。相应地，与 PUFA β-氧化（cpt1和pparα）和合成（fas和elovl5 ）相关的基因的转录水平) 在 D3 和 D4 组中较低。基于WG的回归分析，最佳日粮VE水平为90.75 mg/kg，这与上述结果一致，即饲喂日粮D3和D4的鱼表现出更好的生长性能、抗氧化能力和肌肉PUFA稳定性，其中最后一项表明有影响膳食 VE 对 PUFA β-氧化的影响。