Earth's gravity is essential for maintaining skeletal muscle mass and function in the body. The role of gravity in the myogenic mechanism has been studied with animal experiments in the International Space Station. Recently, gravity-control devices allow to study the effects of gravity on cultured cells on the ground. This study demonstrated that simulated microgravity accelerated aging of human skeletal muscle myoblasts in an in-vitro culture. The microgravity culture induced a significant decrease in cell proliferation and an enlargement of the cytoskeleton and nucleus of cells. Similar changes are often observed in aged myoblasts following several passages. In fact, by the microgravity culture the expression of senescence associated β-Gal was significantly enhanced, and some muscle-specific proteins decreased in the enlarged cells. Importantly, these microgravity effects remained with the cells even after a return to normal gravity conditions. Consequently, the microgravity-affected myoblasts demonstrated a reduced capability of differentiation into myotubes. In the body, it is difficult to interpret the disability of microgravity-affected myoblasts, since muscle regeneration is linked to the supply of new myogenic cells. Therefore, our in-vitro cell culture study will be advantageous to better understand the role of each type of myogenic cell in human muscle without gravitational stress at the single cell level.

地球的重力对于维持体内骨骼肌的质量和功能至关重要。国际空间站的动物实验研究了重力在肌原性机制中的作用。最近，重力控制装置可以研究重力对地面培养细胞的影响。这项研究表明，模拟微重力加速了体外培养中人类骨骼肌成肌细胞的衰老。微重力培养导致细胞增殖显着降低，细胞骨架和细胞核增大。在几代之后，在老化的成肌细胞中经常观察到类似的变化。事实上，通过微重力培养，衰老相关β-Gal的表达显着增强，一些肌肉特异性蛋白在增大的细胞中减少。重要的是，即使在恢复正常重力条件后，这些微重力效应仍然存在于细胞中。因此，受微重力影响的成肌细胞表现出分化为肌管的能力降低。在体内，很难解释受微重力影响的成肌细胞的残疾，因为肌肉再生与新肌原细胞的供应有关。因此，我们的体外细胞培养研究将有利于更好地了解每种类型的肌原细胞在人体肌肉中的作用，而不会在单细胞水平上产生重力应力。很难解释受微重力影响的成肌细胞的残疾，因为肌肉再生与新肌原细胞的供应有关。因此，我们的体外细胞培养研究将有利于更好地了解每种类型的肌原细胞在人体肌肉中的作用，而不会在单细胞水平上产生重力压力。很难解释受微重力影响的成肌细胞的残疾，因为肌肉再生与新肌原细胞的供应有关。因此，我们的体外细胞培养研究将有利于更好地了解每种类型的肌原细胞在人体肌肉中的作用，而不会在单细胞水平上产生重力应力。