Cholangiocarcinoma (CCA) is an aggressive bile duct cancer. Opisthorchis viverrini (O. viverrini) infection is a significant cause of CCA in the Greater Mekong subregion. Currently, there is no standard chemotherapeutic regimen for CCA. A unique hamster carcinogenesis model of O. viverrini–associated CCA was established. Molecular targets identified from the hamster CCA-comparative model are valuable for target identification and validation. Hamster CCA was induced by the administration of O. viverrini metacercariae and N-nitrosodimethylamine. Hamster-derived cancer cells were isolated and continuously cultured for more than 6 months. Ham-2 cell line was established and characterized in vitro and in vivo. Ham-2 exhibited chromosome hyperploidy. A comparative study with previously established cell line, Ham-1, demonstrated that Ham-2 acquired slower growth, higher adhesion, higher migration, and resistance to doxorubicin and 5-fluorouracil (5-FU). In BALB/c Rag-2/Jak3 double-deficient (BRJ) mice, Ham-2 subcutaneous transplantation formed mucin-producing cancers, which morphologically resemble human tubular cholangiocarcinoma. Intravenous-injected Ham-2 established the metastatic nodules in the lungs and livers of BRJ mice. Altogether, a new hamster cholangiocarcinoma cell line, Ham-2, which acquired more aggressive phenotypes in vitro and in vivo, was established. This cell line might be a valuable tool for comparative drug target identification and validation.

胆管癌（CCA）是一种侵袭性胆管癌。Opisthorchis viverrini ( O. viverrini )感染是大湄公河次区域CCA的重要原因。目前，CCA 没有标准的化疗方案。建立了一个独特的O. viverrini相关 CCA仓鼠致癌模型。从仓鼠 CCA 比较模型中确定的分子目标对于目标识别和验证很有价值。仓鼠 CCA 是由O. viverrini metacercariae 和N诱导的。-亚硝基二甲胺。分离仓鼠来源的癌细胞并连续培养6个月以上。Ham-2 细胞系在体外和体内建立和表征。Ham-2 表现出染色体超倍性。与先前建立的细胞系 Ham-1 的比较研究表明，Ham-2 获得了较慢的生长、更高的粘附性、更高的迁移以及对阿霉素和 5-氟尿嘧啶 (5-FU) 的抗性。在 BALB/c Rag-2/Jak3 双缺陷 (BRJ) 小鼠中，Ham-2 皮下移植形成了产生粘蛋白的癌症，其形态类似于人管状胆管癌。静脉注射 Ham-2 在 BRJ 小鼠的肺和肝脏中建立了转移性结节。总之，建立了一种新的仓鼠胆管癌细胞系Ham-2，它在体外和体内获得了更具侵袭性的表型。