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A novel 31bp deletion within the CDKL5 gene is significantly associated with growth traits in Dezhou donkey
Animal Biotechnology ( IF 3.7 ) Pub Date : 2021-09-20 , DOI: 10.1080/10495398.2021.1977653
Fuwen Wang 1 , Gang Wang 1 , Baligen Dalielihan 1 , Zhaofei Wang 1 , Tingjin Chang 1 , Ge Yang 1 , Chuzhao Lei 1 , Ruihua Dang 1
Affiliation  

Abstract

The discovery of molecular markers which associate with livestock economic traits is of great significance for livestock breeding. Selective analysis has found a potential correlation between CDKL5 and growth traits, but there is still a lack of experimental proof. In this study, a 31-bp deletion (g.176595_176626delATGTCACATGTGGTACTGCCATGTGGAATTT) of CDKL5 gene was found by sequencing. The 31-bp indel was then genotyped in 380 individuals of Dezhou donkeys by polyacrylamide gel electrophoresis and there were three genotypes in this population. After the association analysis between growth traits and genotypes, it was found that this 31-bp indel polymorphism was significantly associated with the chest circumference of Dezhou donkeys (p < 0.05), and body length, chest depth and rump width (p < 0.01). In addition, all individuals with DD genotype were better than those with other genotypes in growth traits. This study revealed that a newly identified polymorphic locus in the CDKL5 gene is related to growth traits, which provides a molecular marker for genetic improvement of Dezhou donkey and may lay a solid foundation for the breeding of Dezhou donkey.



中文翻译:

CDKL5基因内的一个新的31bp缺失与德州驴的生长性状显着相关

摘要

发现与畜牧经济性状相关的分子标记对于畜牧育种具有重要意义。选择性分析发现CDKL5与生长性状之间存在潜在相关性,但仍缺乏实验证据。本研究通过测序发现CDKL5基因有一个31 bp的缺失(g.176595_176626delATGTCACATGTGGTACTGCCATGTGGAATTT) 。通过聚丙烯酰胺凝胶电泳对 380 头德州驴进行 31 bp 插入缺失基因分型,发现该群体存在 3 种基因型。经过生长性状与基因型的关联分析发现,这个31 bp的indel多态性与德州驴的胸围显着相关(p < 0.05),以及体长、胸深和臀部宽度 ( p  < 0.01)。此外,所有DD基因型个体的生长性状均优于其他基因型个体。本研究揭示了CDKL5基因中新发现的一个与生长性状相关的多态性位点,为德州驴的遗传改良提供了分子标记,可能为德州驴的选育奠定坚实的基础。

更新日期:2021-09-20
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