Rumen is a natural fermentation system and the microorganisms inside can effectively utilize plant bioresource and interact with host metabolism. Here, analysis of rumen microbiome, together with animal performance and serum metabolism in a lamb model were performed to identify the potential use of mulberry leaf silage (MS) to replace alfalfa silage (AS) as a new functional feed resource and to mining the novel specific mulberry leaf associated rumen bacteria interact with host metabolism. The lambs fed with MS diet showed improved antioxidant capacity and immune function compared to those fed AS diet. The MS diet significantly altered rumen microbiota α- and β-diversity and taxonomic composition. Microbial analysis revealed that Bifidobacterium, Lactobacillus and Schwartzia were enhanced, and Ruminococcaceae UCG-010 and Lachnospiraceae_XPB1014_group were down-regulated in the rumen of MS group. A strong association was also found between these rumen microbial taxa and host antioxidant and immunomodulatory capacity. These findings indicated that mulberry leaf silage can be a high-quality feed source or bioactive pharmaceutical that is responsible for ruminant’s health benefits. The modified rumen microbial community by mulberry leaf silage were associated with the enhanced antioxidant capacity and immunomodulatory of lambs.

中文翻译：

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桑叶青贮对羔羊抗氧化和免疫调节活性及瘤胃细菌群落的影响

瘤胃是一个天然的发酵系统，里面的微生物可以有效地利用植物生物资源并与宿主代谢相互作用。在这里，对瘤胃微生物组以及羔羊模型中的动物性能和血清代谢进行分析，以确定桑叶青贮 (MS) 替代苜蓿青贮 (AS) 作为新的功能性饲料资源的潜在用途，并挖掘新型饲料特定桑叶相关瘤胃细菌与宿主代谢相互作用。与饲喂 AS 日粮的羔羊相比，饲喂 MS 日粮的羔羊表现出更高的抗氧化能力和免疫功能。MS 饮食显着改变了瘤胃微生物群的 α-和 β-多样性和分类组成。微生物分析显示，双歧杆菌、乳酸杆菌和施瓦茨氏菌得到增强，MS组瘤胃中Ruminococcaceae UCG-010和Lachnospiraceae_XPB1014_group表达下调。在这些瘤胃微生物分类群与宿主抗氧化和免疫调节能力之间也发现了强烈的关联。这些发现表明，桑叶青贮饲料可以是一种高质量的饲料来源或生物活性药物，对反刍动物的健康有益。桑叶青贮改良瘤胃微生物群落与羔羊抗氧化能力和免疫调节能力增强有关。这些发现表明，桑叶青贮饲料可以是一种高质量的饲料来源或生物活性药物，对反刍动物的健康有益。桑叶青贮改良瘤胃微生物群落与羔羊抗氧化能力和免疫调节能力增强有关。这些发现表明，桑叶青贮饲料可以是一种高质量的饲料来源或生物活性药物，对反刍动物的健康有益。桑叶青贮改良瘤胃微生物群落与羔羊抗氧化能力和免疫调节能力增强有关。