Some removable medical devices such as catheters and cardiovascular biomaterials require antiadhesive properties towards both prokaryotic and eukaryotic cells in order to prevent the tissues from infections upon implantation and, from alteration upon removal. In order to inhibit cell adhesion, we developed ultrathin hydrated Layer-by-Layer (LbL) coatings composed of biocompatible polyelectrolytes, namely chondroitin sulfate A (CSA) and poly-l-lysine (PLL). The coatings were crosslinked with genipin (GnP), a natural and biocompatible crosslinking agent, to increase their resistance against environmental changes. In order to confer antibacterial activity to the coatings, we proceeded to the electrostatically-driven immobilization of nisin Z, an antimicrobial peptide (AMP) active against gram-positive bacteria. The nisin-enriched coatings had a significantly increased anti-proliferative impact on fibroblasts, as well as a strong contact-killing activity against Staphylococcus aureus in the short and long term.

一些可移除的医疗器械，例如导管和心血管生物材料，需要对原核细胞和真核细胞具有抗粘附特性，以防止组织在植入时受到感染，并在移除时发生改变。为了抑制细胞粘附，我们开发了由生物相容性聚电解质组成的超薄水合逐层 (LbL) 涂层，即硫酸软骨素 A (CSA) 和聚l-赖氨酸 (PLL)。涂层与京尼平 (GnP) 交联，这是一种天然的生物相容性交联剂，以增加它们对环境变化的抵抗力。为了赋予涂层抗菌活性，我们继续静电驱动的乳链菌肽 Z 固定化，乳链菌肽 Z 是一种对革兰氏阳性菌有活性的抗菌肽 (AMP)。富含乳链菌肽的涂层对成纤维细胞的抗增殖作用显着增加，并在短期和长期内对金黄色葡萄球菌具有很强的接触杀伤活性。