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piRNA-independent transposon silencing by the Drosophila THO complex
Developmental Cell ( IF 11.8 ) Pub Date : 2021-09-20 , DOI: 10.1016/j.devcel.2021.08.021
Gen Zhang 1 , Tianxiong Yu 2 , Swapnil S Parhad 1 , Samantha Ho 1 , Zhiping Weng 3 , William E Theurkauf 1
Affiliation  

piRNAs guide Piwi/Panoramix-dependent H3K9me3 chromatin modification and transposon silencing during Drosophila germline development. The THO RNA export complex is composed of Hpr1, Tho2, and Thoc5–7. Null thoc7 mutations, which displace Thoc5 and Thoc6 from a Tho2-Hpr1 subcomplex, reduce expression of a subset of germline piRNAs and increase transposon expression, suggesting that THO silences transposons by promoting piRNA biogenesis. Here, we show that the thoc7-null mutant combination increases transposon transcription but does not reduce anti-sense piRNAs targeting half of the transcriptionally activated transposon families. These mutations also fail to reduce piRNA-guided H3K9me3 chromatin modification or block Panoramix-dependent silencing of a reporter transgene, and unspliced transposon transcripts co-precipitate with THO through a Piwi- and Panoramix-independent mechanism. Mutations in piwi also dominantly enhance germline defects associated with thoc7-null alleles. THO thus functions in a piRNA-independent transposon-silencing pathway, which acts cooperatively with Piwi to support germline development.



中文翻译:

果蝇THO复合物对piRNA独立的转座子沉默

piRNA 在果蝇种系发育过程中指导 Piwi/Panoramix 依赖的 H3K9me3 染色质修饰和转座子沉默。THO RNA 输出复合物由 Hpr1、Tho2 和 Thoc5-7 组成。无效的 thoc7突变从 Tho2-Hpr1 亚复合体中取代 Thoc5 和 Thoc6,降低了生殖系 piRNA 子集的表达并增加了转座子表达,这表明 THO 通过促进 piRNA 生物发生使转座子沉默。在这里,我们展示了thoc7-null 突变组合增加转座子转录,但不减少针对一半转录激活转座子家族的反义 piRNA。这些突变也未能减少 piRNA 引导的 H3K9me3 染色质修饰或阻断报告转基因的 Panoramix 依赖性沉默,并且未剪接的转座子转录物通过 Piwi 和 Panoramix 独立机制与 THO 共沉淀。piwi中的突变也主要增强与thoc7 -null 等位基因相关的种系缺陷。因此,THO 在不依赖 piRNA 的转座子沉默通路中发挥作用,该通路与 Piwi 协同作用以支持种系发育。

更新日期:2021-09-28
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