ABSTRACT

Soil salinization is one of the causes of the decline of the available cultivated land. By improving the salt tolerance of vegetables, the abandoned salinized land can be effectively utilized, saving the arable land for major crops. We investigated the salt tolerance of hundreds of Chinese cabbage cultivars, by which the two most tolerant and sensitive cultivars were selected and studied by metabolome and transcriptome analyses. The results showed that, under salt stress, metabolites’ response was not closely correlated with gene expressions in the tested Chinese cabbages. However, some KEGG pathways were significantly regulated at both metabolic and transcriptional levels. Furthermore, we identified several critical metabolites in the co-regulated pathways, including acetyl-CoA, pyruvic acid, ATP, nicotinic acid and Coenzyme Q10, which could alleviate the salt stress level in the tested Chinese cabbage. Thus, our findings provide candidate agents which can be used to improve salt tolerance in Chinese cabbage.

摘要

土壤盐渍化是可利用耕地减少的原因之一。通过提高蔬菜的耐盐性，可以有效利用废弃的盐渍化土地，节约主要农作物的耕地。我们调查了数百个大白菜品种的耐盐性，从中选择了两个最耐受和最敏感的品种，并通过代谢组和转录组分析进行了研究。结果表明，在盐胁迫下，受试大白菜的代谢物反应与基因表达不密切相关。然而，一些 KEGG 通路在代谢和转录水平上都受到显着调节。此外，我们确定了协同调节途径中的几种关键代谢物，包括乙酰辅酶 A、丙酮酸、ATP、烟酸和辅酶 Q10，可减轻受试大白菜的盐胁迫水平。因此，我们的研究结果提供了可用于提高大白菜耐盐性的候选药物。