Abstract

In this study, ethanol (ETH) was incorporated with glutaraldehyde (GTA) as crosslinking protecting reagent to crosslink as-spun gelatin/pullulan composite nanofibrous membranes at room temperature. The effects of the volume ratios between ETH and GTA on the fibers’ morphology were investigated by scanning electron microscope (SEM). SEM showed the fibrous morphology could be well kept when crosslinked in the ETH/GTA vapor, which illustrates that the ETH played a key role in avoiding the adhesion between fibers. Meanwhile, the thermal properties and mechanical properties of crosslinked membranes were improved significantly after crosslinking. Moreover, the crystallinity of crosslinked membrane improved to 54% compared with 35% of electrospun membrane and the water contact angle of the crosslinked membrane rose to 52.4°, which illustrated both the stability in water and hydrophilicity. Therefore, the crosslinked membranes had good stability and hydrophilicity and thus would be a good candidate as a tissue engineering scaffold.

摘要

在这项研究中，乙醇（ETH）与戊二醛（GTA）作为交联保护剂在室温下交联初生明胶/普鲁兰复合纳米纤维膜。通过扫描电子显微镜（SEM）研究了ETH和GTA的体积比对纤维形态的影响。SEM显示在ETH/GTA蒸气中交联时纤维形态可以很好地保持，这说明ETH在避免纤维之间的粘附方面起着关键作用。同时，交联膜的热性能和力学性能在交联后显着提高。此外，交联膜的结晶度比静电纺膜的 35% 提高到 54%，交联膜的水接触角提高到 52.4°，这说明了在水中的稳定性和亲水性。因此，交联膜具有良好的稳定性和亲水性，是组织工程支架的良好候选者。