Morbilliviruses are amongst the most contagious viral pathogens that infect mammals. Metagenomic surveys have identified numerous morbillivirus sequences in bats, but no full-length authentic morbillivirus has been isolated or characterized from bats. Here we detail the discovery of full-length Myotis Bat Morbillivirus (MBaMV) from a bat surveillance program in Brazil. After determining that MBaMV utilizes bat CD150 but not human CD150 as an entry receptor, we generated an infectious clone of MBaMV using reverse genetics. MBaMV exhibited features consistent with other morbilliviruses, including pleomorphic virions, P-editing and the rule-of-six. MBaMV replicated well in human epithelial cell lines in a nectin-4 dependent manner. Surprisingly, MBaMV was able to infect human macrophages in a CD150-independent manner. However, MBaMV was restricted by cross-neutralizing human sera and did not evade the human innate immune system, indicating that while zoonotic spillover into humans may be possible, MBaMV replication in humans would likely be restricted.

中文翻译：

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评估一种新型蝙蝠麻疹病毒的人畜共患潜力

麻疹病毒是感染哺乳动物的最具传染性的病毒病原体之一。宏基因组调查已经确定了蝙蝠中的大量麻疹病毒序列，但尚未从蝙蝠中分离或表征全长的真实麻疹病毒。在这里，我们详细介绍了从巴西蝙蝠监测计划中发现的全长 Myotis Bat Morbillivirus (MBaMV)。在确定 MBaMV 使用蝙蝠 CD150 而不是人类 CD150 作为进入受体后，我们使用反向遗传学生成了 MBaMV 的感染性克隆。MBaMV 表现出与其他麻疹病毒一致的特征，包括多形性病毒体、P 编辑和六规则。MBaMV 在人上皮细胞系中以 nectin-4 依赖性方式复制良好。令人惊讶的是，MBaMV 能够以不依赖 CD150 的方式感染人类巨噬细胞。然而，