Abstract

Ganoderma lucidum (G. lucidum) is a famous medicinal fungus used as a traditional medicine for generations in China. Among bioactivities that G. lucidum possesses, the anti-tumor effect has aroused extensive interests. In this study, one new triterpene (1) was isolated from 90% ethanol extract of the dried fruiting bodies of G. lucidum. Its structure was determined based on the analysis of its spectroscopic data, including 1 D and 2 D NMR and MS spectra. Furthermore, 1 elicited moderate anti-tumor activities (IC₅₀ values of 15.38 ± 0.34 and 18.61 ± 0.55 μM for A549 and HepG2 cells, respectively) compared with cisplatin which was employed as the positive drug with IC₅₀ values of 8.21 ± 0.17 and 5.36 ± 0.29 μM for A549 and HepG2 cells, respectively. The mechanism study by RT-qPCR and western blot analysis suggested that the p53/caspase-3 pathway is involved in the 1-induced apoptosis.

摘要

灵芝（G. lucidum）是我国历代传统药材的著名药用菌类。在灵芝所具有的生物活性中，其抗肿瘤作用引起了广泛关注。本研究从灵芝干子实体的90%乙醇提取物中分离出一种新的三萜( 1 ) 。其结构是基于对其光谱数据的分析确定的，包括 1 D 和 2 D NMR 和 MS 光谱。此外，与用作具有IC _{50的阳性药物的顺铂相比，} 1引起中等的抗肿瘤活性（A549 和HepG2 细胞的IC _{50值分别为15.38 ± 0.34 和18.61 ± 0.55 μM）}A549 和 HepG2 细胞的值分别为 8.21 ± 0.17 和 5.36 ± 0.29 μM。通过 RT-qPCR 和蛋白质印迹分析的机制研究表明 p53/caspase-3 通路参与了1诱导的细胞凋亡。