A sixty-day feeding trial was conducted to elucidate the effect of coated and uncoated crystalline amino acid mix (CAA) in Penaeus monodon. The control diet (CNT) was formulated with 250 g/kg fishmeal, and three more test diets were prepared by replacing 50% (125 g/kg) of fishmeal (w/w) using a blend of plant proteins with no supplementation of CAA (AA-NL), supplementation of uncoated (AA-NC) and coated (AA-CT) CAA. The AA-CT diet showed (p < .05) lower leaching of amino acids (1.13%) than other diets (2.78%–3.04%). DGC was high (p < .05) in shrimp fed on CNT and AA-CT (1.48%–1.49%/day) than other groups (1.32%–1.37%/day). Of all the treatments, FCR was poor (p < .05) in AA-NL (2.25). The dietary treatments did not influence survival (p = .841) and whole-body composition. The peak of free amino acid was synchronized (60 min) between AA-CT and CNT groups. The control group showed a higher nitrogen intake, oxygen consumption and O:N ratio and lower ammonia-N excretion. Haemolymph indices varied (p < .05) among the dietary groups. As a result of the N-metabolism study, the experimental diets were ranked as CNT>AA-CT>AA-NC. In conclusion, a blend of CAA could be effective in formulating low-fishmeal diets and could replace 50% (125 g/kg) of dietary fishmeal using a plant protein mix in P. monodon.

中文翻译：

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在配制斑节对虾低鱼粉日粮中添加包衣和未包衣的结晶氨基酸混合物（Fabricius，1798）：对生长、消化率、身体成分、血淋巴指数和氮代谢的影响

进行了为期 60 天的喂养试验，以阐明涂层和未涂层​​结晶氨基酸混合物 (CAA) 对斑节对虾的影响。对照日粮 (CNT) 用 250 g/kg 鱼粉配制，另外三种试验日粮通过使用不添加 CAA 的植物蛋白混合物替代 50% (125 g/kg) 鱼粉 (w/w) 制备(AA-NL)，未涂层 (AA-NC) 和涂层 (AA-CT) CAA 的补充。AA-CT 日粮显示 ( p  < .05) 氨基酸浸出 (1.13%) 低于其他日粮 (2.78%–3.04%)。用 CNT 和 AA-CT（1.48%–1.49%/天）喂养的虾的DGC 比其他组（1.32%–1.37%/天）高（p  < .05）。在所有处理中，FCR 较差（p < .05) 在 AA-NL (2.25) 中。饮食治疗不影响生存率 ( p  = .841) 和全身成分。游离氨基酸的峰值在 AA-CT 和 CNT 组之间同步（60 分钟）。对照组显示出较高的氮摄入量、耗氧量和O:N比以及较低的氨氮排泄。不同饮食组的血淋巴指数不同 ( p  < .05)。作为 N 代谢研究的结果，实验日粮的等级为 CNT>AA-CT>AA-NC。总之，CAA 混合物可以有效地配制低鱼粉日粮，并且可以使用P中的植物蛋白混合物替代 50% (125 g/kg) 的日粮鱼粉。 斑节节。