Frontiers in Immunology ( IF 7.3 ) Pub Date : 2021-09-20 , DOI: 10.3389/fimmu.2021.729558 Andriy Trailin 1 , Petra Mrazova 1 , Petra Hruba 1 , Ludek Voska 2 , Eva Sticova 2 , Antonij Slavcev 3 , Marek Novotny 4, 5 , Matej Kocik 6 , Ondrej Viklicky 1, 4
Molecular assessment of renal allografts has already been suggested in antibody-mediated rejection (ABMR), but little is known about the gene transcript patterns in particular renal compartments. We used laser capture microdissection coupled with quantitative RT-PCR to distinguish the transcript patterns in the glomeruli and tubulointerstitium of kidney allografts in sensitized retransplant recipients at high risk of ABMR. The expressions of 13 genes were quantified in biopsies with acute active ABMR, chronic active ABMR, acute tubular necrosis (ATN), and normal findings. The transcripts were either compartment specific (
中文翻译:
慢性活性抗体介导的排斥与间质而非肾小球转录物的上调有关
已经在抗体介导的排斥反应 (ABMR) 中提出了对肾同种异体移植物的分子评估,但对特定肾区室中的基因转录模式知之甚少。我们使用激光捕获显微切割结合定量 RT-PCR 来区分具有高 ABMR 风险的致敏再移植受者的肾小球和肾小管间质的转录模式。在具有急性活动性 ABMR、慢性活动性 ABMR、急性肾小管坏死 (ATN) 和正常结果的活检组织中量化了 13 个基因的表达。转录本是特定于隔室的(