Molecular assessment of renal allografts has already been suggested in antibody-mediated rejection (ABMR), but little is known about the gene transcript patterns in particular renal compartments. We used laser capture microdissection coupled with quantitative RT-PCR to distinguish the transcript patterns in the glomeruli and tubulointerstitium of kidney allografts in sensitized retransplant recipients at high risk of ABMR. The expressions of 13 genes were quantified in biopsies with acute active ABMR, chronic active ABMR, acute tubular necrosis (ATN), and normal findings. The transcripts were either compartment specific (TGFB1 in the glomeruli and HAVCR1 and IGHG1 in the tubulointerstitium), ABMR specific (GNLY), or follow-up specific (CXCL10 and CX3CR1). The transcriptional profiles of early acute ABMR shared similarities with ATN. The transcripts of CXCL10 and TGFB1 increased in the glomeruli in both acute ABMR and chronic active ABMR. Chronic active ABMR was associated with the upregulation of most genes (SH2D1B, CX3CR1, IGHG1, MS4A1, C5, CD46, and TGFB1) in the tubulointerstitium. In this study, we show distinct gene expression patterns in specific renal compartments reflecting cellular infiltration observed by conventional histology. In comparison with active ABMR, chronic active ABMR is associated with increased transcripts of tubulointerstitial origin.

已经在抗体介导的排斥反应 (ABMR) 中提出了对肾同种异体移植物的分子评估，但对特定肾区室中的基因转录模式知之甚少。我们使用激光捕获显微切割结合定量 RT-PCR 来区分具有高 ABMR 风险的致敏再移植受者的肾小球和肾小管间质的转录模式。在具有急性活动性 ABMR、慢性活动性 ABMR、急性肾小管坏死 (ATN) 和正常结果的活检组织中量化了 13 个基因的表达。转录本是特定于隔室的（TGFB1 在肾小球和 HAVCR1 和 温室气体1 在肾小管间质中），ABMR 特异性（格力)，或后续特定的 (CXCL10 和 CX3CR1）。早期急性 ABMR 的转录谱与 ATN 有相似之处。的成绩单CXCL10 和 TGFB1在急性 ABMR 和慢性活动性 ABMR 的肾小球中均增加。慢性活动性 ABMR 与大多数基因的上调有关（SH2D1B, CX3CR1, 温室气体1, MS4A1, C5, CD46， 和 TGFB1) 在肾小管间质中。在这项研究中，我们显示了特定肾区室中不同的基因表达模式，反映了常规组织学观察到的细胞浸润。与活动性 ABMR 相比，慢性活动性 ABMR 与肾小管间质起源的转录物增加有关。