Lung cancer (LC) is a malignant tumor with the highest incidence and mortality rates worldwide. Linc00284, a long non-coding RNA, is a newly discovered regulator of LC. This study aimed to explore the role of Linc00284 in LC progression. Gene expression levels were detected by RT-qPCR and/or western blot analysis. Cell migratory and invasive capabilities were measured by wound healing and transwell assays. Subcutaneous xenograft models were constructed to examine tumor growth of LC cells. Data showed that Linc00284 was significantly upregulated in LC tissues compared to adjacent normal lung tissues and predicted poor prognosis in patients with LC. In vitro, Linc00284 was highly expressed in LC cells and was mainly localized in the cytoplasm. Mechanistically, Linc00284 directly bound to miR-205-3p, leading to the upregulation of c-Met expression. A significant negative correlation was observed between Linc00284 and miR-205-3p expression levels, and the Linc00284 level was positively correlated with the c-Met expression. Linc00284/miR-205-3p/c-Met regulatory axis promotes LC cell proliferation, migration, and invasion. Furthermore, the in vivo results indicated that Linc00284 knockdown markedly suppressed tumor growth. Taken together, these data suggest that Linc00284 facilitates LC progression by targeting the miR-205-3p/c-Met axis, which may be a potential target for LC treatment.

肺癌（LC）是全球发病率和死亡率最高的恶性肿瘤。Linc00284 是一种长链非编码 RNA，是新发现的 LC 调控因子。本研究旨在探讨 Linc00284 在 LC 进展中的作用。通过 RT-qPCR 和/或蛋白质印迹分析检测基因表达水平。细胞迁移和侵袭能力是通过伤口愈合和 transwell 测定来测量的。构建皮下异种移植模型以检查 LC 细胞的肿瘤生长。数据显示，与邻近的正常肺组织相比，Linc00284 在 LC 组织中显着上调，并预测 LC 患者的预后不良。体外, Linc00284 在 LC 细胞中高表达，主要定位于细胞质中。从机制上讲，Linc00284 直接与 miR-205-3p 结合，导致 c-Met 表达上调。观察到 Linc00284 与 miR-205-3p 表达水平呈显着负相关，Linc00284 水平与 c-Met 表达呈正相关。Linc00284/miR-205-3p/c-Met 调节轴促进 LC 细胞增殖、迁移和侵袭。此外，该体内结果表明，敲低 Linc00284 显着抑制了肿瘤生长。总之，这些数据表明 Linc00284 通过靶向 miR-205-3p/c-Met 轴促进 LC 进展，这可能是 LC 治疗的潜在靶点。