Two new Cu (II) complexes [Cu (bpy)₂L1] BF₄.CH₃OH (Z₃) and [Cu (phen)₂L1] BF₄·H₂O (Z9), L1 = 5-Fluorouracil-1-yl Acetic Acid, were synthesized based on 5-Fluorouracil-1-yl Acetic Acid and 2,2′-Bipyridine or 1,10-phenanthroline ligands and their anticancer activity toward human cancer cell lines investigated. The complexes were characterized by infrared (IR) spectra, elemental analysis, and X-ray crystallography. The interaction of the complexes with CT-DNA was investigated by UV–vis absorption and fluorescence spectroscopies, and cyclic voltammetry; cell viability (%) was studied using the absorbance amount of the samples. The interaction mode of the complexes with DNA is electrostatic, and the complexes displayed good anticancer activity against HCT 116 (human colorectal cancer cells) and MDA-MB-231 (MD Anderson-metastatic breast) cell lines with best IC₅₀ amount of 11.31 ± 0.74 μM for Z9. The results showed that the nature of the nitrogen-donor ligand is very effective in the anticancer activity of the complexes.

中文翻译：

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基于 5-fluorouracil-1-yl 乙酸和 N-供体配体的两种新型 Cu (II) 配合物：研究它们与 DNA 的相互作用和抗癌活性

两种新的 Cu (II) 配合物 [Cu (bpy) ₂ L1] BF ₄ .CH ₃ OH (Z ₃ ) 和 [Cu (phen) ₂ L1] BF ₄ ·H ₂O (Z9), L1 = 5-Fluorouracil-1-yl Acetic Acid，是基于 5-Fluorouracil-1-yl Acetic Acid 和 2,2'-Bipyridine 或 1,10-phenanthroline 配体及其对人的抗癌活性合成的研究了癌细胞系。通过红外 (IR) 光谱、元素分析和 X 射线晶体学对配合物进行了表征。通过紫外-可见吸收光谱和荧光光谱以及循环伏安法研究了配合物与 CT-DNA 的相互作用；使用样品的吸光度来研究细胞活力 (%)。配合物与DNA的相互作用方式是静电的，配合物对HCT 116（人结直肠癌细胞）和MDA-MB-231（MD Anderson-转移性乳腺）细胞系表现出良好的抗癌活性，IC _50最好Z9 的量为 11.31 ± 0.74 μM。结果表明，氮供体配体的性质对配合物的抗癌活性非常有效。