The dried aerial parts of the plant Artemisia glabella Kar. et Kir. contain about 1.1% of the very important sesquiterpene lactone arglabin. This compound can be extracted by supercritical CO₂ giving a 20 time concentrated extract containing about 22% arglabin. The CO₂ extract can be efficiently treated by hydrostatic countercurrent chromatography (CCC) able to produce in a single run 95% pure arglabin. Both mobile and stationary phase are liquid in CCC. The biphasic waterless liquid system heptane:ethyl acetate:acetonitrile 2:1:2 v/v/v was used to separate arglabin from its two major co-extracted compounds: argolide and eucalyptol. A three-factor Box–Behnken design was used to optimize the CCC preparative purification of the CO₂ extract by a FCPC-5L instrument. Injected amount, mobile phase flow rate, and rotor rotation speed were used to optimize arglabin productivity. An optimized run was able to treat 90 g of extract producing 20 g of 95% pure arglabin in 50 min, a theoretical productivity of 400 mg/min.

植物Artemisia glabella Kar的干燥地上部分。和基尔。含有约 1.1% 的非常重要的倍半萜内酯 arglabin。该化合物可以通过超临界 CO ₂提取，得到 20 倍浓缩提取物，其中含有约 22% arglabin。CO ₂提取物可以通过静水逆流色谱 (CCC) 进行有效处理，能够在单次运行中产生 95% 纯度的 arglabin。流动相和固定相在 CCC 中都是液体。双相无水液体系统庚烷:乙酸乙酯:乙腈 2:1:2 v/v/v 用于将 arglabin 与其两种主要的共提取化合物分离：argolide 和桉叶油素。三因素 Box-Behnken 设计用于优化 CO ₂的 CCC 制备纯化FCPC-5L 仪器提取。进样量、流动相流速和转子转速用于优化 arglabin 生产率。优化运行能够在 50 分钟内处理 90 克提取物，产生 20 克纯度为 95% 的 arglabin，理论生产率为 400 毫克/分钟。