We present a facile approach for the direct attachment of cross-linked poly(2-thiopheneacetic acid) onto the surface of magnetite nanoparticles with one single carbon layer (Fe₃O₄@1C NPs) via self-assembly oxidative polymerization. The functional Fe₃O₄@1C NPs combined with poly(2-thiopheneacetic acid) [Fe₃O₄@1C@ poly(Th) NPs] was successfully prepared and used for magnetic solid-phase extraction (MSPE). The influence of the preparation conditions and extraction conditions were investigated in detail. The morphology and size distribution of the monolithic materials were characterized by transmission electron microscopy. Under the optimized experimental conditions, the prepared Fe₃O₄@1C@ poly(Th) NPs showed good extraction efficiency for canthin-6-one alkaloids. Furthermore, the results indicated that the binding specificity and selectivity of the Fe₃O₄@1C@ poly(Th) NPs might be attributed to electrostatic interactions and pore structure. A MSPE–HPLC method was also developed for quantitative analysis of canthin-6-one alkaloids. The developed method showed good linearity, low limit of detections, low limit of quantifications, and good reproducibility over a wide linear range. To further prove the utility, Fe₃O₄@1C@ poly(Th) NPs-MSPE was also used to extract canthin-6-one alkaloids from Eurycoma longifolia stems, with a recovery rate of 87.9–114%. Thus, this method is potentially applicable for the extraction of similar alkaloid compounds from medicinal herb samples.

我们提出了一种通过自组装氧化聚合将交联聚（2-噻吩乙酸）直接连接到具有单碳层（Fe ₃ O ₄ @1C NPs）的磁铁矿纳米颗粒表面的简便方法。功能性 Fe ₃ O ₄ @1C NPs 与聚（2-噻吩乙酸）[Fe ₃ O ₄ @1C@poly(Th) NPs] 组合成功制备并用于磁性固相萃取 (MSPE)。详细研究了制备条件和提取条件的影响。通过透射电子显微镜表征整体材料的形态和尺寸分布。在优化的实验条件下，制备的 Fe₃ O ₄ @1C@poly(Th) NPs 对角蛋白-6-one 生物碱显示出良好的提取效率。此外，结果表明 Fe ₃ O ₄ @1C@poly(Th) NPs的结合特异性和选择性可能归因于静电相互作用和孔结构。还开发了一种 MSPE-HPLC 方法来定量分析角蛋白 6-one 生物碱。开发的方法显示出良好的线性、检测下限、定量下限以及在宽线性范围内的良好重现性。为了进一步证明其效用，Fe ₃ O ₄ @1C@poly(Th) NPs-MSPE 也被用于从Eurycoma longifolia 中提取 canthin-6-one 生物碱茎，回收率为 87.9-114%。因此，该方法可能适用于从药草样品中提取类似的生物碱化合物。