Strigolactones (SLs) are a class of phytohormones playing diverse roles in plant growth and development, yet the limited access to SLs is largely impeding SL-based foundational investigations and applications. Here, we developed Escherichia coli–Saccharomyces cerevisiae consortia to establish a microbial biosynthetic platform for the synthesis of various SLs, including carlactone, carlactonoic acid, 5-deoxystrigol (5DS; 6.65 ± 1.71 μg/liter), 4-deoxyorobanchol (3.46 ± 0.28 μg/liter), and orobanchol (OB; 19.36 ± 5.20 μg/liter). The SL-producing platform enabled us to conduct functional identification of CYP722Cs from various plants as either OB or 5DS synthase. It also allowed us to quantitatively compare known variants of plant SL biosynthetic enzymes in the microbial system. The titer of 5DS was further enhanced through pathway engineering to 47.3 μg/liter. This work provides a unique platform for investigating SL biosynthesis and evolution and lays the foundation for developing SL microbial production process.

中文翻译：

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独脚金内酯生产菌-酵母联合体的建立

独脚金内酯 (SLs) 是一类在植物生长和发育中发挥多种作用的植物激素，但对 SLs 的有限获取在很大程度上阻碍了基于 SLs 的基础研究和应用。在这里，我们开发了大肠杆菌——酿酒酵母联合体建立微生物生物合成平台，用于合成各种 SL，包括 carlactone、carlactonoic acid、5-deoxystrigol (5DS; 6.65 ± 1.71 μg/L)、4-deoxyorobanchol (3.46 ± 0.28 μg/L) 和 orobanchol (OB ; 19.36 ± 5.20 微克/升）。SL 生产平台使我们能够对来自各种植物的 CYP722Cs 作为 OB 或 5DS 合酶进行功能鉴定。它还使我们能够定量比较微生物系统中植物 SL 生物合成酶的已知变体。通过通路工程，5DS 的滴度进一步提高到 47.3 μg/L。这项工作为研究 SL 生物合成和进化提供了一个独特的平台，并为开发 SL 微生物生产工艺奠定了基础。