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Optimization of hyphenated asymmetric flow field-flow fractionation for the analysis of silver nanoparticles in aqueous solutions
Analytical and Bioanalytical Chemistry ( IF 4.3 ) Pub Date : 2021-09-19 , DOI: 10.1007/s00216-021-03647-3
Felix Geißler 1 , María Martínez-Cabanas 1 , Pablo Lodeiro 1, 2 , Eric P Achterberg 1
Affiliation  

The extensive use of silver nanoparticles (AgNPs) in consumer products, medicine, and industry leads to their release into the environment. Thus, a characterization of the concentration, size, fate, and toxicity of AgNPs under environmental conditions is required. In this study, we present the characterization and optimization of an asymmetric flow field-flow fractionation (AF4) system coupled with UV/Vis spectrophotometer and dynamic light scattering (DLS) detector as a powerful tool for the size separation and multi-parameter characterization of AgNPs in complex matrices. The hyphenated AF4-UV/Vis-DLS system was first characterized using individual injections of the different size fractions. We used electrostatically stabilized AgNPs of 20-, 50-, and 80-nm nominal diameters coated with lipoic acid. We investigated the effect of applied cross-flows, carrier solutions, focus times, and quantity of injected particles on the nature of the AF4 fractograms and on the integrity of the AgNPs. Best size separation of a 1:1 mixture of 20- and 80-nm AgNPs was achieved using cross-flows of 0.5 and 0.7 mL/min with 1 mM NaCl and 0.05% v/v Mucasol as carrier solutions. We also researched the behavior of AgNPs in natural waters using the hyphenated AF4-UV/Vis-DLS system, under determined optimal conditions.

Graphical abstract



中文翻译:

用于分析水溶液中银纳米粒子的联用不对称流场-流分级优化

银纳米粒子 (AgNPs) 在消费品、医药和工业中的广泛使用导致它们释放到环境中。因此,需要在环境条件下对 AgNPs 的浓度、大小、归宿和毒性进行表征。在本研究中,我们介绍了不对称流场-流分馏 (AF4) 系统的表征和优化,该系统与紫外/可见分光光度计和动态光散射 (DLS) 检测器相结合,作为尺寸分离和多参数表征的有力工具。复杂基质中的 AgNP。联用 AF4-UV/Vis-DLS 系统首先使用不同尺寸级分的单独注射来表征。我们使用涂有硫辛酸的 20、50 和 80 nm 标称直径的静电稳定 AgNPs。我们研究了应用的交叉流、载体溶液、聚焦时间和注入粒子的数量对 AF4 分形图的性质和 AgNPs 完整性的影响。以 1 mM NaCl 和 0.05% v/v Mucasol 作为载体溶液,使用 0.5 和 0.7 mL/min 的交叉流实现了 20-和 80-nm AgNPs 的 1:1 混合物的最佳尺寸分离。我们还使用联用 AF4-UV/Vis-DLS 系统在确定的最佳条件下研究了 AgNPs 在天然水中的行为。

图形概要

更新日期:2021-09-19
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