Rotarix^® vaccine was implemented nationwide in Zambia in 2013. In this study, four unusual strains collected in the post-vaccine period were subjected to whole genome sequencing and analysis. The four strains possessed atypical genotype constellations, with at least one reassortant genome segment within the constellation. One of the strains (UFS-NGS-MRC-DPRU4749) was genetically and phylogenetically distinct in the VP4 and VP1 gene segments. Pairwise analyses demonstrated several amino acid disparities in the VP4 antigenic sites of this strain compared to that of Rotarix^®. Although the impact of these amino acid disparities remains to be determined, this study adds to our understanding of the whole genomes of reassortant strains circulating in Zambia following Rotarix^® vaccine introduction.

中文翻译：

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人类轮状病毒的全基因组分析揭示了赞比亚的单基因重组轮状病毒株

Rotarix ^®疫苗于 2013 年在赞比亚全国实施。在这项研究中，在疫苗接种后期间收集的四种不寻常菌株进行了全基因组测序和分析。这四种菌株具有非典型基因型星座，星座内至少有一个重配基因组片段。其中一种菌株 (UFS-NGS-MRC-DPRU4749) 在 VP4 和 VP1 基因片段中在遗传和系统发育上是不同的。^{配对分析表明，与Rotarix ®}相比，该菌株的VP4 抗原位点存在几个氨基酸差异。尽管这些氨基酸差异的影响仍有待确定，但这项研究增加了我们对 Rotarix ^{®后在赞比亚流行的重配菌株全基因组的理解}疫苗介绍。