Although many phytochemicals have been used in traditional medicine, there is a great need to refresh the health benefits and adjust the shortcomings of herbal medicine. In this research, two herbal principles (Diosgenin and Glycyrrhiza glabra extract) coopted in the Nanostructured Lipid Carriers have been developed for improving the most desirable properties of herbal medicine—antioxidant and anti-inflammatory actions. The contribution of phytochemicals, vegetable oils and of lipid matrices has been highlighted by comparative study of size, stability, entrapment efficiency, morphological characteristics, and thermal behavior. According to the in vitro MTS and RTCA results, the dual herbal-NLCs were no cytotoxic toward endothelial cells at concentrations between 25 and 100 µg/mL. A rapid release of Glycyrrhiza glabra and a motivated delay of Diosgenin was detected by the in vitro release experiments. Dual herbal-NLCs showed an elevated ability to annihilate long-life cationic radicals (ABTS^•+) and short-life oxygenated radicals (an inhibition of 63.4% ABTS^•+, while the ability to capture radical oxygen species reached 96%). The production of pro-inflammatory cytokines was significantly inhibited by the newly herbals-NLC (up to 97.9% inhibition of TNF-α and 62.5% for IL-6). The study may open a new pharmacotherapy horizon; it provides a comprehensive basis for the use of herbal-NLC in the treatment of inflammatory diseases.

中文翻译：

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双重草药原理负载脂质纳米载体在提供高治疗效果方面的多方面作用

虽然传统医学中已经使用了许多植物化学物质，但非常需要刷新健康益处并调整草药的缺点。在这项研究中，开发了纳米结构脂质载体中的两种草药成分（薯蓣皂苷元和光果甘草提取物），以改善草药最理想的特性——抗氧化和抗炎作用。通过对尺寸、稳定性、截留效率、形态特征和热行为的比较研究，突出了植物化学物质、植物油和脂质基质的贡献。根据体外 MTS 和 RTCA 结果，双草药 NLC 在 25 和 100 µg/mL 之间的浓度下对内皮细胞没有细胞毒性。快速释放体外释放实验检测到甘草和薯蓣皂苷元的有动机延迟。双草本-NLC 显示出更高的消灭长寿命阳离子自由基 (ABTS ^•+ ) 和短寿命氧化自由基的能力（抑制 63.4% ABTS ^•+，同时捕获自由基氧物种的能力达到 96%）。新草药-NLC 显着抑制了促炎细胞因子的产生（高达 97.9% 的 TNF-α 抑制和 62.5% 的 IL-6）。该研究可能会开辟一个新的药物治疗视野；它为草药-NLC在炎症性疾病的治疗中的应用提供了全面的基础。