Abstract

The effect of exposure to acute hypoxia on the level of methemoglobin (MtHb) in blood and the activity of catalase (CAT) and superoxide dismutase (SOD) in erythrocytes of the Black Scorpionfish (Scorpaena porcus, Linnaeus 1758) was studied in experiments in vivo. The O₂ concentration in seawater was reduced from 8.5–8.7 mgO₂/L to the required values by pumping N₂ into the water for 1.5–2.0 h. The concentration range of 0.35–8.50 mgO₂/L was studied. The exposure lasted for 4 h at a water temperature of 14–16°C. This showed that at oxygen concentrations in water less than 2 mgO₂/L part of hemoglobin passes into the ferric form. The effect was most pronounced in the case of acute hypoxia exposure (less than 1 mgO₂/L). The maximum increase in the MtHb level was 19% of the total level of the pigment in blood. This correlated with a significant increase in the SOD and CAT activity in erythrocytes (with the coefficient of determination R² higher than 0.750) and indicated an increase in the dismutation of superoxide anion in red blood cells.

中文翻译：

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暴露于急性缺氧的黑蝎子鱼 (Scorpaena porcus, Linnaeus 1758) 红细胞中的过氧化氢酶和超氧化物歧化酶活性以及血液中的高铁血红蛋白水平

摘要

在体内实验中研究了急性缺氧暴露对黑蝎鱼( Scorpaena porcus , Linnaeus 1758)血液中高铁血红蛋白 (MtHb) 水平以及过氧化氢酶 (CAT) 和超氧化物歧化酶 (SOD)活性的影响. 通过将 N ₂泵入水中 1.5-2.0 小时，海水中的 O ₂浓度从 8.5-8.7 mgO ₂ /L降低到所需值。研究了 0.35–8.50 mgO ₂ /L的浓度范围。在 14-16°C 的水温下暴露 4 小时。这表明，当水中的氧浓度低于 2 mgO ₂/L 部分血红蛋白转化为三价铁形式。在急性缺氧暴露（低于 1 mgO ₂ /L）的情况下，效果最为明显。MtHb 水平的最大增加是血液中色素总水平的 19%。这与红细胞中 SOD 和 CAT 活性的显着增加相关（决定系数R ²高于 0.750），并表明红细胞中超氧阴离子的歧化增加。