Abstract

Hemolymph is one of the main tissues providing invertebrates’ immunity and homeostasis. Earlier, we showed that some of the hemolymph immune parameters of the common Far Eastern mollusk Modiolus kurilensis are sensitive to bacterial and anthropogenic challenges. In this work, a number of cellular parameters such as total hemocyte count (THC), hemocyte phagocytic activity (PA), and reactive oxygen species (ROS) and humoral parameters such as hemagglutination (HA) and hemolytic (HL) activity of plasma, as well as its protein concentration (C_P) and profiling in M. kurilensis were studied depending on the season and environmental indicators (temperature, salinity, and oxygen concentration). The total hemocyte count was weakly correlated with water oxygen concentration (R = –0.25) and temperature (R = 0.27), and the 36 kDa plasma protein was weakly correlated with temperature (R = –0.33). Also, there was a correlation (R = 0.38) between hemagglutination activity and the 19 kDa protein fraction. The minimum values for a number of parameters were noted in January (THC, PA, ROS, HA, HL), while the maximum values were observed in late spring (THC, PA, ROS, HA, HL). At the same time, in other months, these and most other parameters had metastable values. Thus, the indicators that were identified in previous studies (THC, PA, ROS, HA, HL, C_P, protein fractions 55 and 124 kDa) are suitable for assessing the physiological state of M. kurilensis during most of the year.

中文翻译：

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1983年日本海东方湾双壳类Modiolus kurilensis Bernard血淋巴参数的季节性变化

摘要

血淋巴是提供无脊椎动物免疫和体内平衡的主要组织之一。早些时候，我们表明，常见的远东软体动物Modiolus kurilensis 的一些血淋巴免疫参数对细菌和人为挑战很敏感。在这项工作中，一些细胞参数，如总血细胞计数 (THC)、血细胞吞噬活性 (PA) 和活性氧 (ROS) 和体液参数，如血浆的血凝 (HA) 和溶血 (HL) 活性，以及它的蛋白质浓度 ( C _P ) 和在M. kurilensis 中的分析根据季节和环境指标（温度、盐度和氧气浓度）进行研究。总血细胞计数与水氧浓度 ( R = –0.25) 和温度 ( R = 0.27) 呈弱相关，36 kDa 血浆蛋白与温度 ( R = –0.33)呈弱相关。此外，存在相关性（R= 0.38) 血凝活性和 19 kDa 蛋白质级分之间。许多参数的最小值出现在 1 月份（THC、PA、ROS、HA、HL），而最大值出现在晚春（THC、PA、ROS、HA、HL）。同时，在其他月份，这些和大多数其他参数都具有亚稳态值。因此，在以前的研究（THC，PA，ROS，HA，HL，中确定的指标Ç _P，蛋白级分55和124 kDa的）是合适的用于评估的生理状态M. kurilensis期间在一年的大部分。