The long-followed practice of using garlic cloves for garlic asexual reproduction has resulted in virus accumulation and genetic depression. Aerial bulbs, the preferred material for virus-free garlic seedling cultivation in vitro, undergo dormancy, but gibberellin can quickly break this state. In this study, exogenous GA₃ was applied to dormant aerial bulbs and transcriptome sequencing was then performed using control and gibberellin-treated aerial bulbs. Genes differentially expressed in response to the gibberellin treatment were analyzed and screened and their relationship to GA₃-induced dormancy break of aerial bulbs was explored. According to our results, exogenous GA₃ was able to effectively break aerial bulb dormancy. Transcriptome sequencing uncovered 4931 and 74,636 genes that were respectively up- and down-regulated in response to gibberellin treatment. 37,957 differentially expressed genes were successfully assigned to KEGG pathways, among which the most DEGs were annotated in metabolism pathway (23,245 genes, accounting for 61.24%), followed by the genetic information processing pathway (9235 genes, accounting for 24.33%). More DEGs were enriched in spliceosome, ribosome, ribosome biogenesis in eukaryotes, and ubiquitin-mediated proteolysis pathways and the enrichment results were significant. In addition, carbohydrate and amino acid metabolism pathways are active. The significant DEGs related to calcium signaling, hormonal signaling, and transcription factors were screened out. Among them, the expression of DEGs related to hormonal signaling was consistent with the change of endogenous hormone content in aerial bulbs. Finally, the outcome of a qRT-PCR analysis was consistent with our transcriptome sequencing results, thus indicating that the latter was reliable.

长期使用大蒜瓣进行大蒜无性繁殖的做法导致病毒积累和遗传抑制。球茎是大蒜体外无病毒育苗的首选材料，会休眠，但赤霉素可以迅速打破这种状态。在这项研究中，外源 GA ₃被应用于休眠的空中鳞茎，然后使用对照和赤霉素处理的空中鳞茎进行转录组测序。分析和筛选响应赤霉素处理而差异表达的基因，并探索它们与GA ₃诱导的气生球茎休眠破坏的关系。根据我们的结果，外源 GA ₃能够有效打破空中灯泡休眠。转录组测序发现了 4931 和 74,636 个基因，它们分别响应赤霉素处理而上调和下调。37,957个差异表达基因被成功分配到KEGG通路，其中DEGs在代谢通路中注释最多（23,245个基因，占61.24%），其次是遗传信息处理通路（9235个基因，占24.33%）。更多的DEGs在真核生物的剪接体、核糖体、核糖体生物发生和泛素介导的蛋白水解途径中富集，富集结果显着。此外，碳水化合物和氨基酸代谢途径是活跃的。筛选出与钙信号、激素信号和转录因子相关的重要 DEG。他们之中，与激素信号相关的DEGs的表达与气生球茎内源激素含量的变化一致。最后，qRT-PCR 分析的结果与我们的转录组测序结果一致，表明后者是可靠的。