Enterovirus 71 (EV71) poses a major threat to public health globally due to severe and even fatal hand, foot, and mouth disease (HFMD). However, no effective antiviral agents are available to treat HFMD caused by this virus. Polysaccharides have been shown to exhibit antiviral activity, and polysaccharides extracted from Picochlorum sp. 122 (PPE) could potentially be used to treat HFMD, but reports on their antiviral activity are limited. In this study, the antiviral activity of PPE against EV71 was verified in Vero cells. PPE was shown to limit EV71 infection, as demonstrated using an MTT assay and by observing the cellular cytopathic effect. In addition, a decrease in VP1 RNA and protein levels indicated that PPE effectively inhibits proliferation of EV71 in Vero cells. An annexin V affinity assay also indicated that PPE protects host cells from apoptosis through the AKT and ATM/ATR signalling pathways. These results demonstrate that PPE has potential as an antiviral drug to treat HFMD caused by EV71.

中文翻译：

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从 Picochlorum sp. 中提取的多糖对肠道病毒 71 感染的抑制作用。122 通过 AKT 和 ATM/ATR 信号通路。

由于严重甚至致命的手足口病 (HFMD)，肠道病毒 71 (EV71) 对全球公共卫生构成重大威胁。然而，没有有效的抗病毒药物可用于治疗由该病毒引起的手足口病。多糖已显示出抗病毒活性，而多糖是从 Picochlorum sp. 中提取的。122 (PPE) 可能用于治疗手足口病，但有关其抗病毒活性的报道有限。在这项研究中，PPE 对 EV71 的抗病毒活性在 Vero 细胞中得到验证。PPE 被证明可以限制 EV71 感染，如使用 MTT 测定和观察细胞致细胞病变效应所证明的那样。此外，VP1 RNA 和蛋白质水平的降低表明 PPE 有效抑制了 Vero 细胞中 EV71 的增殖。膜联蛋白 V 亲和力测定还表明，PPE 通过 AKT 和 ATM/ATR 信号通路保护宿主细胞免于凋亡。这些结果表明，PPE 具有作为治疗 EV71 引起的手足口病的抗病毒药物的潜力。