Archives of Virology ( IF 2.7 ) Pub Date : 2021-09-18 , DOI: 10.1007/s00705-021-05235-3 Xia Chen 1 , Du Hai 2 , Jincang Li 2 , Jie Tan 1 , Shuping Huang 1 , Hongyuan Zhang 1 , Hao Chen 1 , Min Zhang 1
Here, we report the discovery and molecular characterization of a novel mitovirus isolated from tissues of Lagenaria siceraria, named "Lagenaria siceraria associated mitovirus 1" (LsaMV1). Next-generation sequencing and adapter-ligation-mediated amplification were used to obtain the full-length genome sequence of LsaMV1. The genome of LsaMV1 is 3,098 nucleotides (nt) long and contains an opening reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRP). Homology searches and phylogenetic analysis of the 855-aa RdRP suggested that LsaMV1 is a member of a new species in the family Mitoviridae. This is the first report of a member of the family Mitoviridae associated with the important summer vegetable bottle gourd.
中文翻译:
一种与 Lagenaria siceraria 相关的新型有丝分裂病毒的完整基因组序列
在这里,我们报告了从Lagenaria siceraria组织中分离出的一种新型有丝分裂病毒的发现和分子特征,该病毒被命名为“Lagenaria siceraria 相关有丝分裂病毒 1”(LsaMV1)。使用二代测序和接头连接介导的扩增获得 LsaMV1 的全长基因组序列。LsaMV1 的基因组长 3,098 个核苷酸 (nt),包含一个开放阅读框 (ORF),编码推定的 RNA 依赖性 RNA 聚合酶 (RdRP)。855-aa RdRP 的同源性搜索和系统发育分析表明,LsaMV1 是线粒体病毒科新物种的成员。这是与重要的夏季蔬菜葫芦有关的线粒体病毒科成员的第一份报告。