In this study, the regulation effects of selenium (Se) on the expression of pyrin domain-containing protein (NLRP) 3 inflammasome and reactive oxygen species (ROS) in bovine mammary epithelial cells (bMECs) infected by Staphylococcus aureus (S. aureus) were detected. bMECs were treated with 8 μmol/L Na2SeO3 for 12 h before infection with S. aureus for 2 h. Through flow cytometry, Western blot, and qRT-PCR analysis, the expression of ROS and NLRP3 imflammasome was detected. Results showed Se significantly reduced the ROS level in bMECs; at the same time, the expressions of NLRP3, ASC, caspase-1, Pro-IL-1β, and IL-1β were also decreased. In conclusion, Se inhibits S. aureus-induced inflammation by suppressing the activation of NLRP3 inflammasome and ROS in bMECs.

中文翻译：

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硒通过调节 ROS 诱导的 NLRP3 炎症小体改善金黄色葡萄球菌诱导的牛乳腺上皮细胞炎症。

本研究探讨硒（Se）对金黄色葡萄球菌（S. aureus）感染牛乳腺上皮细胞（bMECs）中pyrin结构域蛋白（NLRP）3炎症小体和活性氧（ROS）表达的调节作用被检测到。bMECs 用 8 μmol/L Na2SeO3 处理 12 小时，然后用金黄色葡萄球菌感染 2 小时。通过流式细胞仪、Western blot和qRT-PCR分析检测ROS和NLRP3炎症小体的表达。结果表明，硒显着降低了 bMEC 中的 ROS 水平；同时，NLRP3、ASC、caspase-1、Pro-IL-1β、IL-1β的表达也降低。总之，硒通过抑制 bMEC 中 NLRP3 炎性体和 ROS 的激活来抑制金黄色葡萄球菌诱导的炎症。