A total of two red oleaginous yeasts, Rhodotorula diobovata and Rhodosporidium babjevae, were investigated for their potential to grow on nitrogen-limited media with sufficient glycerol as carbon source and produce biomass, triacylglycerides (TAGs) and carotenoids. The two yeasts produced equal quantities of biomass by 120 h post-inoculation (h pi), but R. diobovata consumed more glycerol than R. babajavae under the same conditions. The TAG concentrations accumulated by R. diobovata and R. babjevae were greater than 20% dry cell weight (dcw), and the major fatty acid components consisted of palmitic acid, oleic acid and linolenic acid. The highest concentration of total fatty acids in biomass were present during the late of stationary phase were 486.3 mg/g dcw for R. diobovata at 120 h pi, and 243.9 mg/g dcw for R. babjevae at 144 h pi. Both R. diobovata and R. babjevae produced high concentrations of torularhodin, and low amounts of torulene and γ-carotene. Total carotenoid concentrations in R. diobovata biomass were 31.5 mg/g dcw at 120 h pi and 43.1 mg/g dcw at 96 h pi for R. babjevae. The dcw accumulations of carotenoids by R. diobovata and R. babjevae were significantly greater than those reported for other carotenogenic Rhodotorula and Rhodosporidium strains.

中文翻译：

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在以甘油为唯一碳源的限氮条件下培养的红酵母和巴氏红孢的中性脂质和类胡萝卜素的积累

研究了总共两种红色产油酵母Rhodotorula diobovata和Rhodosporidium babjevae，它们在以足够的甘油作为碳源的限氮培养基上生长并产生生物量、三酰基甘油酯 (TAGs) 和类胡萝卜素的潜力。两个酵母由120小时后接种（H PI）产生的生物量的数量相等，但R. diobovata消耗更多甘油比R. babajavae相同的条件下。R. diobovata和R. babjevae积累的 TAG 浓度大于 20% 干细胞重量 (dcw)，主要脂肪酸成分由棕榈酸、油酸和亚麻酸组成。生物质中总脂肪酸的最高浓度出现在固定相后期，R. diobovata在 120 h pi 为486.3 mg/g dcw，R. babjevae在 144 h pi为 243.9 mg/g dcw 。既R. diobovata和R. babjevae产生高浓度的torularhodin，和少量torulene和γ胡萝卜素。R. diobovata生物量中的总类胡萝卜素浓度在120 小时后为 31.5 毫克/克 dcw，而在 96 小时后对于R. babjevae为 43.1 mg/g dcw。通过类胡萝卜素的DCW积累R. diobovata和R. babjevae显着大于其他类胡萝卜素红酵母和红孢子菌菌株所报道的那些。