Periodontitis is defined as a multifactorial polymicrobial infection accompanied by inflammatory reactions. Porphyromonas gingivalis (Pg) is known as a major pathogen in the initiation and progression of periodontitis, and a major virulence factor is Pg lipopolysaccharide (LPS). Molecular hydrogen (H₂) has been reported to act as a gaseous antioxidant, which suppresses periodontitis progression by decreasing gingival oxidative stress. However, no human periodontitis model has examined the anti-inflammatory effects of H₂. In this study, we examined the effects of H₂ on Pg LPS-induced secretion of 8 types of inflammation markers in a human periodontitis model using human gingival cells with enzyme-linked immunosorbent assays. Our results demonstrated that Pg LPS increased interleukin (IL) 1 alpha (IL-1α) and IL-6 secretion, but H₂ significantly suppressed the secretion of both cytokines without cytotoxicity. H₂ can suppress the production of IL-1α and IL-6, which are identified as cytokines involved in inflammatory reactions in periodontal disease. Thus, H₂ may provide therapeutic applications for periodontitis.

牙周炎被定义为伴有炎症反应的多因素多微生物感染。牙龈卟啉单胞菌（Pg）是牙周炎发生和发展的主要病原体，主要毒力因子是Pg脂多糖（LPS）。据报道，分子氢 (H ₂ ) 可作为气态抗氧化剂，通过降低牙龈氧化应激来抑制牙周炎的进展。_{然而，没有人类牙周炎模型检测过H 2}的抗炎作用。在这项研究中，我们检查了 H ₂对Pg的影响LPS 在人牙周炎模型中诱导 8 种炎症标志物的分泌，使用人牙龈细胞进行酶联免疫吸附试验。我们的结果表明，Pg LPS 增加了白细胞介素 (IL) 1 α (IL-1α) 和 IL-6 的分泌，但 H ₂显着抑制了这两种细胞因子的分泌，而没有细胞毒性。H ₂可以抑制IL-1α和IL-6的产生，它们被确定为参与牙周病炎症反应的细胞因子。因此，H ₂可以为牙周炎提供治疗应用。