The crosstalk between tumor and stroma plays a critical role in cancer metastasis. However, the function of miR-10a-5p on liver fibroblasts in the metastatic microenvironment of colon cancer (CC) and the effect of activated fibroblasts on CC cells are still unclear. In our study, miR-10a-5p overexpression inhibited the proliferation, migration, and IL-6/IL-8 level of LX-2 and human liver cancer fibroblasts (HLCFs) cells. Moreover, miR-10a-5p had lower expression in HLCFs than in human liver normal fibroblasts (HLNFs). The conditioned medium (CM) from LX-2 cells with miR-10a-5p overexpression and HLNFs could inhibit the invasion, migration, and stemness of CC SW480 cells, whereas HLCFs CM could promote these malignant phenotypes of SW480 cells. The present study illustrates the effect of miR-10a-5p on the liver fibroblasts and the altered liver fibroblasts in the microenvironment on CC cells induced by miR-10a-5p, which may aid the understanding of the mechanisms underlying CC liver metastasis.

中文翻译：

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microRNA-10a-5p 过表达通过调节人肝癌成纤维细胞来抑制结肠癌的恶性肿瘤。

肿瘤和基质之间的串扰在癌症转移中起关键作用。然而，miR-10a-5p对结肠癌（CC）转移微环境中肝成纤维细胞的作用以及活化成纤维细胞对CC细胞的影响仍不清楚。在我们的研究中，miR-10a-5p 过表达抑制了 LX-2 和人肝癌成纤维细胞 (HLCFs) 细胞的增殖、迁移和 IL-6/IL-8 水平。此外，miR-10a-5p 在 HLCFs 中的表达低于人肝正常成纤维细胞 (HLNFs)。来自具有 miR-10a-5p 过表达和 HLNFs 的 LX-2 细胞的条件培养基 (CM) 可以抑制 CC SW480 细胞的侵袭、迁移和干性，而 HLCFs CM 可以促进 SW480 细胞的这些恶性表型。