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Silencing of a Pseudo-nitzschia arenysensis lipoxygenase transcript leads to reduced oxylipin production and impaired growth
New Phytologist ( IF 9.4 ) Pub Date : 2021-09-17 , DOI: 10.1111/nph.17739
Valeria Sabatino 1 , Ida Orefice 1 , Pina Marotta 1 , Luca Ambrosino 1 , Maria Luisa Chiusano 1, 2 , Giuliana d'Ippolito 3 , Giovanna Romano 1 , Angelo Fontana 3, 4 , Maria Immacolata Ferrante 1
Affiliation  

  • Because of their importance as chemical mediators, the presence of a rich and varied family of lipoxygenase (LOX) products, collectively named oxylipins, has been investigated thoroughly in diatoms, and the involvement of these products in important processes such as bloom regulation has been postulated. Nevertheless, little information is available on the enzymes and pathways operating in these protists.
  • Exploiting transcriptome data, we identified and characterized a LOX gene, PaLOX, in Pseudo-nitzschia arenysensis, a marine diatom known to produce different species of oxylipins by stereo- and regio-selective oxidation of eicosapentaenoic acid (EPA) at C12 and C15.
  • PaLOX RNA interference correlated with a decrease of the lipid-peroxidizing activity and oxylipin synthesis, as well as with a reduction of growth of P. arenysensis. In addition, sequence analysis and structure models of the C-terminal part of the predicted protein closely fitted with the data for established LOXs from other organisms.
  • The presence in the genome of a single LOX gene, whose downregulation impairs both 12- and 15-oxylipins synthesis, together with the in silico 3D protein modelling suggest that PaLOX encodes for a 12/15S-LOX with a dual specificity, and provides additional support to the correlation between cell growth and oxylipin biosynthesis in diatoms.


中文翻译:

Pseudo-nitzschia arenysensis 脂氧合酶转录物的沉默导致氧化脂产生减少和生长受损

  • 由于它们作为化学介质的重要性,已在硅藻中彻底研究了丰富多样的脂氧合酶 (LOX) 产物家族(统称为氧脂素)的存在,并假设这些产物参与重要的过程,如开花调节. 然而,关于在这些原生生物中运作的酶和途径的信息很少。
  • 利用转录组数据,我们在Pseudo-nitzschia arenysensis 中鉴定并表征了 LOX 基因PaLOX,这是一种已知通过在 C12 和 C15 处对二十碳五烯酸 (EPA) 进行立体和区域选择性氧化而产生不同种类的氧化脂的海洋硅藻。
  • PaLOX RNA 干扰与脂质过氧化活性和氧化脂合成的降低以及与P. arenysensis生长的减少相关。此外,预测蛋白质的 C 末端部分的序列分析和结构模型与来自其他生物的已建立 LOX 的数据非常吻合。
  • 基因组中存在单个LOX基因,其下调会损害 12-和 15-oxylipins 合成,以及计算机3D 蛋白质建模表明PaLOX编码具有双重特异性的 12/15 S -LOX,并提供进一步支持了硅藻中细胞生长和氧化脂生物合成之间的相关性。
更新日期:2021-09-17
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