Preclinical tests for evaluating potential drug candidates using conventional protocols can be exhaustive and high-cost processes. Microfluidic technologies that can speed up this process and allow fast screening of drugs are promising alternatives. This work presents the design, concept, and operational conditions of a simple, modular, and reversible sealing microdevice useful for drug screening. This microdevice allows for the operation of 4 parallel simultaneous conditions and can also generate a diffusive concentration gradient in sextuplicates. We used laminated polydimethylsiloxane (PDMS_LAM) and glass as building materials as proof of concept. The PDMS_LAM parts can be reused since they can be easily sterilized. We cultured MCF-7 (Michigan Cancer Foundation-7) breast cancer cells. Cells were exposed to a doxorubicin diffusive concentration gradient for 3 h. They were monitored by automated microscopy, and after data processing, it was possible to determine cell viability as a function of doxorubicin concentration. The reversible sealing enabled the recovery of the tested cells and image acquisition. Therefore, this microdevice is a promising tool for drug screening that allows assessing the cellular behavior in dynamic conditions and the recovery of cells for afterward processing and imaging.

使用常规方案评估潜在候选药物的临床前测试可能是详尽且高成本的过程。可以加速这一过程并允许快速筛选药物的微流体技术是有前途的替代品。这项工作介绍了用于药物筛选的简单、模块化和可逆密封微型装置的设计、概念和操作条件。该微型装置允许同时运行 4 个平行条件，并且还可以产生六份的扩散浓度梯度。我们使用层压聚二甲基硅氧烷 (PDMS _LAM ) 和玻璃作为建筑材料作为概念证明。PDMS _LAM零件可以重复使用，因为它们可以很容易地消毒。我们培养了 MCF-7（密歇根癌症基金会-7）乳腺癌细胞。细胞暴露于多柔比星扩散浓度梯度 3 小时。它们通过自动显微镜进行监测，经过数据处理后，可以确定细胞活力与阿霉素浓度的函数关系。可逆密封使被测细胞的恢复和图像采集成为可能。因此，这种微型装置是一种很有前途的药物筛选工具，可以评估动态条件下的细胞行为以及用于后续处理和成像的细胞恢复。