Rapid detection and surveillance of cfiA-positive Bacteroides fragilis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry,Anaerobe

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Rapid detection and surveillance of cfiA-positive Bacteroides fragilis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry
Anaerobe ( IF 2.3 ) Pub Date : 2021-09-17 , DOI: 10.1016/j.anaerobe.2021.102448
Yasuhide Kawamoto ₁ , Kosuke Kosai ₁ , Kenji Ota ₁ , Naoki Uno ₂ , Kei Sakamoto ₂ , Hiroo Hasegawa ₁ , Koichi Izumikawa ₃ , Hiroshi Mukae ₄ , Katsunori Yanagihara ₅

Affiliation

Objectives

To perform surveillance of cfiA-positive Bacteroides fragilis using new subtyping software module, MALDI Biotyper Subtyping Module (MBT Subtyping Module), on MALDI-TOF MS system, and to evaluate the detection ability of the module.

Methods

cfiA-positive strains were presumed using the module against B. fragilis isolated between 2006 and 2019. The cfiA gene was confirmed using PCR. In cfiA-positive B. fragilis, the insertion sequence (IS) elements were examined and the MBT STAR-BL assay was performed to examine meropenem hydrolysis activity.

Results

Of the 396 B. fragilis strains included, the MBT Subtyping Module detected 33 presumptive cfiA-positive strains (8.3%), of which 32 harbored the cfiA gene. The sensitivity and specificity of the MBT Subtyping Module for detecting cfiA-positive B. fragilis were 100.0% and 99.7%, respectively. Of the 32 strains harboring the cfiA gene, seven strains possessed IS elements, which were thought to induce high cfiA expression. Meropenem hydrolysis was detected in all seven strains that were positive for both cfiA and IS elements, and they exhibited resistance to meropenem and imipenem. The overall non-susceptibility rates to meropenem and imipenem were 84.8% and 36.4%, respectively, in the 33 presumptive cfiA-positive strains.

Conclusion

The MBT Subtyping Module can detect cfiA-positive B. fragilis rapidly and accurately, supporting its use for surveillance of cfiA-positive B. fragilis in clinical settings.

中文翻译：

使用基质辅助激光解吸电离飞行时间质谱法快速检测和监测 cfiA 阳性脆弱拟杆菌

目标

在 MALDI-TOF MS 系统上使用新的分型软件模块 MALDI Biotyper Subtyping Module (MBT Subtyping Module)对cfiA阳性脆弱拟杆菌进行监测，并评估该模块的检测能力。

方法

使用针对2006 年至 2019 年间分离的脆弱拟杆菌的模块推测cfiA阳性菌株。使用 PCR 确认了cfiA基因。在cfiA阳性脆弱拟杆菌中，检查插入序列 (IS) 元件并进行 MBT STAR-BL 测定以检查美罗培南水解活性。

结果

在包括的 396个脆弱拟杆菌菌株中，MBT 分型模块检测到 33 个假定的 cfiA阳性菌株（8.3%），其中 32 个含有cfiA基因。MBT 分型模块检测cfiA阳性脆弱拟杆菌的敏感性和特异性分别为 100.0% 和 99.7%。在含有cfiA基因的 32 株菌株中，7 株具有被认为可诱导高cfiA表达的 IS 元件。在所有 7 株cfiA均呈阳性的菌株中检测到美罗培南水解和IS元素，对美罗培南和亚胺培南表现出耐药性。在 33 种假定的cfiA阳性菌株中，对美罗培南和亚胺培南的总体不敏感率分别为 84.8% 和 36.4% 。