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Increased sHLA-G Is Associated with Improved COVID-19 Outcome and Reduced Neutrophil Adhesion
Viruses ( IF 5.818 ) Pub Date : 2021-09-17 , DOI: 10.3390/v13091855
Daria Bortolotti 1 , Valentina Gentili 1 , Sabrina Rizzo 1 , Giovanna Schiuma 1 , Silvia Beltrami 1 , Savino Spadaro 2 , Giovanni Strazzabosco 1 , Gianluca Campo 3 , Edgardo D Carosella 4 , Alberto Papi 5, 6 , Roberta Rizzo 1, 7 , Marco Contoli 5, 6
Affiliation  

Human leukocyte antigen (HLA) is a group of molecules involved in inflammatory and infective responses. We evaluated blood sHLA-E and sHLA-G levels in hospitalized COVID-19 patients with respiratory failure and their relationship with clinical evolution, changes in endothelial activation biomarker profile, and neutrophil adhesion. sHLA-E, sHLA-G, and endothelial activation biomarkers were quantified by ELISA assay in plasma samples. Neutrophil adhesion to endothelium was assessed in the presence/absence of patients’ plasma samples. At admission, plasma levels of sHLA-G and sHLA-E were significantly higher in COVID-19 patients with respiratory failure compared to controls. COVID-19 clinical improvement was associated with increased sHLA-G plasma levels. In COVID-19, but not in control patients, an inverse correlation was found between serum sICAM-1 and E-selectin levels and plasma sHLA-G values. The in vitro analysis of activated endothelial cells confirmed the ability of HLA-G molecules to control sICAM-1 and sE-selectin expression via CD160 interaction and FGF2 induction and consequently neutrophil adhesion. We suggest a potential role for sHLA-G in improving COVID-19 patients’ clinical condition related to the control of neutrophil adhesion to activated endothelium.

中文翻译:

sHLA-G 增加与改善 COVID-19 结果和减少中性粒细胞粘附有关

人类白细胞抗原 (HLA) 是一组参与炎症和感染反应的分子。我们评估了住院 COVID-19 呼吸衰竭患者的血液 sHLA-E 和 sHLA-G 水平及其与临床演变、内皮活化生物标志物谱的变化和中性粒细胞粘附的关系。sHLA-E、sHLA-G 和内皮活化生物标志物通过 ELISA 测定在血浆样品中定量。在患者血浆样品存在/不存在的情况下评估嗜中性粒细胞对内皮的粘附。入院时,与对照组相比,COVID-19 呼吸衰竭患者的血浆 sHLA-G 和 sHLA-E 水平显着升高。COVID-19 临床改善与 sHLA-G 血浆水平升高有关。在 COVID-19 中,但不是在对照患者中,发现血清 sICAM-1 和 E-选择素水平与血浆 sHLA-G 值之间呈负相关。对活化内皮细胞的体外分析证实了 HLA-G 分子通过 CD160 相互作用和 FGF2 诱导以及随后的中性粒细胞粘附来控制 sICAM-1 和 sE-选择素表达的能力。我们建议 sHLA-G 在改善 COVID-19 患者临床状况方面的潜在作用,与控制中性粒细胞对活化内皮的粘附有关。
更新日期:2021-09-17
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