Porcine astrovirus type 3 (PoAstV3) has been previously identified as a cause of polioencephalomyelitis in swine and continues to cause disease in the US swine industry. Herein, we describe the characterization of both untranslated regions, frameshifting signal, putative genome-linked virus protein (VPg) and conserved antigenic epitopes of several novel PoAstV3 genomes. Twenty complete coding sequences (CDS) were obtained from 32 diagnostic cases originating from 11 individual farms/systems sharing a nucleotide (amino acid) percent identity of 89.74–100% (94.79–100%), 91.9–100% (96.3–100%) and 90.71–100% (93.51–100%) for ORF1a, ORF1ab and ORF2, respectively. Our results indicate that the 5′UTR of PoAstV3 is highly conserved highlighting the importance of this region in translation initiation while their 3′UTR is moderately conserved among strains, presenting alternative configurations including multiple putative protein binding sites and pseudoknots. Moreover, two predicted conserved antigenic epitopes were identified matching the 3′ termini of VP27 of PoAstV3 USA strains. These epitopes may aid in the design and development of vaccine components and diagnostic assays useful to control outbreaks of PoAstV3-associated CNS disease. In conclusion, this is the first analysis predicting the structure of important regulatory motifs of neurotropic mamastroviruses, which differ from those previously described in human astroviruses.

中文翻译：

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美国新型猪星状病毒3型毒株比较分析

猪星状病毒 3 型 (PoAstV3) 先前已被确定为猪脊髓灰质炎的病因，并继续在美国养猪业引起疾病。在这里，我们描述了几个新型 PoAstV3 基因组的非翻译区、移码信号、假定的基因组相关病毒蛋白 (VPg) 和保守抗原表位的表征。从来自 11 个个体农场/系统的 32 个诊断病例中获得 20 个完整编码序列 (CDS)，其核苷酸（氨基酸）百分比同一性为 89.74-100% (94.79-100%)、91.9-100% (96.3-100%) ) 和 ORF1a、ORF1ab 和 ORF2 分别为 90.71–100% (93.51–100%)。我们的结果表明 PoAstV3 的 5'UTR 高度保守，突出了该区域在翻译起始中的重要性，而它们的 3'UTR 在菌株中适度保守，提出替代配置，包括多个推定的蛋白质结合位点和假结。此外，鉴定出两个预测的保守抗原表位与 PoAstV3 USA 菌株的 VP27 的 3' 末端相匹配。这些表位可能有助于设计和开发有助于控制 PoAstV3 相关 CNS 疾病爆发的疫苗成分和诊断分析。总之，这是第一个预测嗜神经性乳腺病毒的重要调控基序结构的分析，这些结构不同于之前在人类星状病毒中描述的那些。这些表位可能有助于设计和开发有助于控制 PoAstV3 相关 CNS 疾病爆发的疫苗成分和诊断分析。总之，这是第一个预测嗜神经性乳腺病毒重要调控基序结构的分析，这些结构不同于之前在人类星状病毒中描述的那些。这些表位可能有助于设计和开发有助于控制 PoAstV3 相关 CNS 疾病爆发的疫苗成分和诊断分析。总之，这是第一个预测嗜神经性乳腺病毒重要调控基序结构的分析，这些结构不同于之前在人类星状病毒中描述的那些。